Induction of maternal immune activation in mice at mid-gestation stage with viral mimic poly(I:C)

Ke Huan Chow, Zihao Yan, Wei Li Wu

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

Maternal immune activation (MIA) model is increasingly well appreciated as a rodent model for the environmental risk factor of various psychiatric disorders. Numerous studies have demonstrated that MIA model is able to show face, construct, and predictive validity that are relevant to autism and schizophrenia. To model MIA, investigators often use viral mimic polyinosinic: polycytidylic acid (poly(I:C)) to activate the immune system in pregnant rodents. Generally, the offspring from immune activated dam exhibit behavioral abnormalities and physiological alterations that are associated with autism and schizophrenia. However, poly(I:C) injection with different dosages and at different time points could lead to different outcomes by perturbing brain development at different stages. Here we provide a detailed method of inducing MIA by intraperitoneal (i.p.) injection of 20 mg/kg poly(I:C) at mid-gestational embryonic 12.5 days (E12.5). This method has been shown to induce acute inflammatory response in the maternal-placental-fetal axis, which ultimately results in the brain perturbations and behavioral phenotypes that are associated with autism and schizophrenia.

原文English
文章編號e53643
期刊Journal of Visualized Experiments
2016
發行號109
DOIs
出版狀態Published - 2016 3月 25

All Science Journal Classification (ASJC) codes

  • 一般神經科學
  • 一般化學工程
  • 一般生物化學,遺傳學和分子生物學
  • 一般免疫學和微生物學

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