Influence of DNA Sequence on the Nature of Mispairing during DNA Synthesis

M. D. Lai, K. L. Beattie

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

A series of synthetic oligonucleotide primers, annealed at various positions along the lacZ-a region of bacteriophage M13mp9 template, were elongated by purified DNA polymerases in the presence of only 3 of the 4 deoxynucleoside triphosphates to achieve misincorporation at a total of 49 different positions along the template. The newly synthesized strands (containing misincorporated bases) were isolated and sequenced to determine the identity of misincorporated deoxynucleoside monophosphates. The results indicate that the kind of mispairing that occurs during DNA synthesis is greatly influenced by the nucleotide sequence of the template. Transition-type base substitutions predominated overall, but at many template positions, transversion-type base substitutions occurred, most commonly via A.A mispairing. The results of parallel determinations made with Escherichia coli DNA polymerase I (“large fragment” form) and DNA polymerase of Maloney murine leukemia virus indicated that, overall, the identity of polymerase had only a small effect on the kind of misincorporation that occurred at different positions along the template. However, at certain template positions, the nature of mispairing during DNA synthesis was reproducibly affected by differing polymerase active-site environment.

原文English
頁(從 - 到)1722-1728
頁數7
期刊Biochemistry
27
發行號5
DOIs
出版狀態Published - 1988 三月 1

All Science Journal Classification (ASJC) codes

  • 生物化學

指紋

深入研究「Influence of DNA Sequence on the Nature of Mispairing during DNA Synthesis」主題。共同形成了獨特的指紋。

引用此