TY - JOUR
T1 - Influence of Spin Coating and Dip Coating with Gelatin/Hydroxyapatite for Bioresorbable Mg Alloy Orthopedic Implants
T2 - In Vitro and In Vivo Studies
AU - Tran, Duong Thuy
AU - Chen, Fang Hsu
AU - Wu, Guan Lin
AU - Ching, Paula Carmela O.
AU - Yeh, Ming Long
N1 - Funding Information:
This research was supported by the Biomaterials and Regeneration Medicine Innovation Lab, Department of BME, National Cheng Kung University. We thank and acknowledge Dr. Chih-Han Lin from the Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan, Dr. Cheng-Lin Wu from the Department of Pathology, and Dr. Tsung-Lin Tsai from the Department of Oncology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan, for helpful discussions and excellent technical support. This research was financially supported by the National Science and Technology Council in Taiwan through Grants MOST 110-2314-B-006-028-MY2.
Funding Information:
This research was supported by the Biomaterials and Regeneration Medicine Innovation Lab, Department of BME, National Cheng Kung University. We thank and acknowledge Dr. Chih-Han Lin from the Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan, Dr. Cheng-Lin Wu from the Department of Pathology, and Dr. Tsung-Lin Tsai from the Department of Oncology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan, for helpful discussions and excellent technical support.
Funding Information:
This research was financially supported by the National Science and Technology Council in Taiwan through Grants MOST 110-2314-B-006-028-MY2.
Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/2/13
Y1 - 2023/2/13
N2 - Suitable biomechanical properties, good biocompatibility, and osteoconductivity of a degradable magnesium (Mg) alloy make it a potential material for orthopedic implants. The main limitation of Mg is its high corrosion rate in the human body. Surface modification is necessary to improve the Mg corrosion resistance. In this work, a polymeric layer of gelatin/nanohydroxyapatite (Gel/nHA) was coated on a ZK60 Mg alloy by dip coating and spin coating to test the corrosion resistance and biocompatibility in vitro and in vivo. The results from the in vitro test revealed that the coated groups reduced the corrosion rate with the corrosion current density by 59 and 81%, from 31.22 to 12.83 μA/cm2 and 5.83 μA/cm2 in the spin coating and dip coating groups, respectively. The dip coating group showed better corrosion resistance than the spin coating group with the lowest released hydrogen content (17.5 mL) and lowest pH value (8.23) and reducing the current density by 45%. In vitro, the relative growth rate was over 75% in all groups tested with MG63, demonstrating that the Mg substrate and coating materials were within the safety range. The dip coating and spin coating groups enhanced the cell proliferation with significantly higher OD values (3.3, 3.0, and 2.5, respectively) and had better antihemolysis and antiplatelet adhesion abilities than the uncoated group. The two coating methods showed no difference in the cellular response, cell migration, hemolysis, and platelet adhesion test. In in vivo tests in rats, the dip coating group also showed a higher corrosion resistance with a lower corrosion rate and mass loss than the spin coating group. In addition, the blood biochemistry and histopathology results indicated that all materials used in this study were biocompatible with living subjects. The present research confirmed that the two methods have no noticeable difference in cell and organ response but the corrosion resistance of dip coating was higher than that of spin coating either in vitro or in vivo.
AB - Suitable biomechanical properties, good biocompatibility, and osteoconductivity of a degradable magnesium (Mg) alloy make it a potential material for orthopedic implants. The main limitation of Mg is its high corrosion rate in the human body. Surface modification is necessary to improve the Mg corrosion resistance. In this work, a polymeric layer of gelatin/nanohydroxyapatite (Gel/nHA) was coated on a ZK60 Mg alloy by dip coating and spin coating to test the corrosion resistance and biocompatibility in vitro and in vivo. The results from the in vitro test revealed that the coated groups reduced the corrosion rate with the corrosion current density by 59 and 81%, from 31.22 to 12.83 μA/cm2 and 5.83 μA/cm2 in the spin coating and dip coating groups, respectively. The dip coating group showed better corrosion resistance than the spin coating group with the lowest released hydrogen content (17.5 mL) and lowest pH value (8.23) and reducing the current density by 45%. In vitro, the relative growth rate was over 75% in all groups tested with MG63, demonstrating that the Mg substrate and coating materials were within the safety range. The dip coating and spin coating groups enhanced the cell proliferation with significantly higher OD values (3.3, 3.0, and 2.5, respectively) and had better antihemolysis and antiplatelet adhesion abilities than the uncoated group. The two coating methods showed no difference in the cellular response, cell migration, hemolysis, and platelet adhesion test. In in vivo tests in rats, the dip coating group also showed a higher corrosion resistance with a lower corrosion rate and mass loss than the spin coating group. In addition, the blood biochemistry and histopathology results indicated that all materials used in this study were biocompatible with living subjects. The present research confirmed that the two methods have no noticeable difference in cell and organ response but the corrosion resistance of dip coating was higher than that of spin coating either in vitro or in vivo.
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U2 - 10.1021/acsbiomaterials.2c01122
DO - 10.1021/acsbiomaterials.2c01122
M3 - Article
C2 - 36695051
AN - SCOPUS:85147100420
SN - 2373-9878
VL - 9
SP - 705
EP - 718
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 2
ER -