Inhibition of corneal angiogenesis by local application of vasostatin

Pei Chang Wu, Lin Cheng Yang, Hsi Kung Kuo, Chao Cheng Huang, Chia Ling Tsai, Pey Ru Lin, Ping-Ching Wu, Shyi Jang Shin, Ming Hong Tai

研究成果: Article

30 引文 (Scopus)

摘要

Purpose: This study was designed to investigate the effects of the locally supplied endogenous angiogenesis inhibitor vasostatin (VS) on corneal angiogenesis. Methods: Recombinant VS was expressed and purified. The effects of VS on the proliferation of endothelial cells were investigated using the methyl thiazolyl tetrazolium (MTT) assay in the absence or presence of angiogenic factors such as basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF). Corneal neovascularization was induced by implantation of hydron pellets containing bFGF in rat corneal micropockets. The potency of VS to inhibit corneal angiogenesis was investigated by incorporation of VS with bFGF in hydron pellets or topical application of VS containing eye drops to rat eyes implanted with bFGF pellets. The extent of corneal neovascularization was evaluated by microscopic and histological analyses. Results: VS potently inhibited the growth of endothelial cells in the absence or presence of angiogenic factors such as bFGF or VEGF. In the rat corneal micropocket assay, concurrent incorporation of VS abolished the bFGF induced neovascularization. When formulated in a methylcellulose eye drop, VS remained intact and functional in a 4 °C solution for more than 7 days. Topical application of VS eye drops potently inhibited bFGF induced neovascularization in rat corneas. Conclusions: The present study effectively demonstrated the potential feasibility of local application of VS for treatment of corneal angiogenesis.

原文English
頁(從 - 到)28-35
頁數8
期刊Molecular Vision
11
出版狀態Published - 2005 一月 13

指紋

Corneal Neovascularization
Fibroblast Growth Factor 2
Ophthalmic Solutions
Angiogenesis Inducing Agents
Vascular Endothelial Growth Factor A
vasostatin
Endothelial Cells
Methylcellulose
Angiogenesis Inhibitors
Cornea

All Science Journal Classification (ASJC) codes

  • Ophthalmology

引用此文

Wu, P. C., Yang, L. C., Kuo, H. K., Huang, C. C., Tsai, C. L., Lin, P. R., ... Tai, M. H. (2005). Inhibition of corneal angiogenesis by local application of vasostatin. Molecular Vision, 11, 28-35.
Wu, Pei Chang ; Yang, Lin Cheng ; Kuo, Hsi Kung ; Huang, Chao Cheng ; Tsai, Chia Ling ; Lin, Pey Ru ; Wu, Ping-Ching ; Shin, Shyi Jang ; Tai, Ming Hong. / Inhibition of corneal angiogenesis by local application of vasostatin. 於: Molecular Vision. 2005 ; 卷 11. 頁 28-35.
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abstract = "Purpose: This study was designed to investigate the effects of the locally supplied endogenous angiogenesis inhibitor vasostatin (VS) on corneal angiogenesis. Methods: Recombinant VS was expressed and purified. The effects of VS on the proliferation of endothelial cells were investigated using the methyl thiazolyl tetrazolium (MTT) assay in the absence or presence of angiogenic factors such as basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF). Corneal neovascularization was induced by implantation of hydron pellets containing bFGF in rat corneal micropockets. The potency of VS to inhibit corneal angiogenesis was investigated by incorporation of VS with bFGF in hydron pellets or topical application of VS containing eye drops to rat eyes implanted with bFGF pellets. The extent of corneal neovascularization was evaluated by microscopic and histological analyses. Results: VS potently inhibited the growth of endothelial cells in the absence or presence of angiogenic factors such as bFGF or VEGF. In the rat corneal micropocket assay, concurrent incorporation of VS abolished the bFGF induced neovascularization. When formulated in a methylcellulose eye drop, VS remained intact and functional in a 4 °C solution for more than 7 days. Topical application of VS eye drops potently inhibited bFGF induced neovascularization in rat corneas. Conclusions: The present study effectively demonstrated the potential feasibility of local application of VS for treatment of corneal angiogenesis.",
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Wu, PC, Yang, LC, Kuo, HK, Huang, CC, Tsai, CL, Lin, PR, Wu, P-C, Shin, SJ & Tai, MH 2005, 'Inhibition of corneal angiogenesis by local application of vasostatin', Molecular Vision, 卷 11, 頁 28-35.

Inhibition of corneal angiogenesis by local application of vasostatin. / Wu, Pei Chang; Yang, Lin Cheng; Kuo, Hsi Kung; Huang, Chao Cheng; Tsai, Chia Ling; Lin, Pey Ru; Wu, Ping-Ching; Shin, Shyi Jang; Tai, Ming Hong.

於: Molecular Vision, 卷 11, 13.01.2005, p. 28-35.

研究成果: Article

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AU - Wu, Pei Chang

AU - Yang, Lin Cheng

AU - Kuo, Hsi Kung

AU - Huang, Chao Cheng

AU - Tsai, Chia Ling

AU - Lin, Pey Ru

AU - Wu, Ping-Ching

AU - Shin, Shyi Jang

AU - Tai, Ming Hong

PY - 2005/1/13

Y1 - 2005/1/13

N2 - Purpose: This study was designed to investigate the effects of the locally supplied endogenous angiogenesis inhibitor vasostatin (VS) on corneal angiogenesis. Methods: Recombinant VS was expressed and purified. The effects of VS on the proliferation of endothelial cells were investigated using the methyl thiazolyl tetrazolium (MTT) assay in the absence or presence of angiogenic factors such as basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF). Corneal neovascularization was induced by implantation of hydron pellets containing bFGF in rat corneal micropockets. The potency of VS to inhibit corneal angiogenesis was investigated by incorporation of VS with bFGF in hydron pellets or topical application of VS containing eye drops to rat eyes implanted with bFGF pellets. The extent of corneal neovascularization was evaluated by microscopic and histological analyses. Results: VS potently inhibited the growth of endothelial cells in the absence or presence of angiogenic factors such as bFGF or VEGF. In the rat corneal micropocket assay, concurrent incorporation of VS abolished the bFGF induced neovascularization. When formulated in a methylcellulose eye drop, VS remained intact and functional in a 4 °C solution for more than 7 days. Topical application of VS eye drops potently inhibited bFGF induced neovascularization in rat corneas. Conclusions: The present study effectively demonstrated the potential feasibility of local application of VS for treatment of corneal angiogenesis.

AB - Purpose: This study was designed to investigate the effects of the locally supplied endogenous angiogenesis inhibitor vasostatin (VS) on corneal angiogenesis. Methods: Recombinant VS was expressed and purified. The effects of VS on the proliferation of endothelial cells were investigated using the methyl thiazolyl tetrazolium (MTT) assay in the absence or presence of angiogenic factors such as basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF). Corneal neovascularization was induced by implantation of hydron pellets containing bFGF in rat corneal micropockets. The potency of VS to inhibit corneal angiogenesis was investigated by incorporation of VS with bFGF in hydron pellets or topical application of VS containing eye drops to rat eyes implanted with bFGF pellets. The extent of corneal neovascularization was evaluated by microscopic and histological analyses. Results: VS potently inhibited the growth of endothelial cells in the absence or presence of angiogenic factors such as bFGF or VEGF. In the rat corneal micropocket assay, concurrent incorporation of VS abolished the bFGF induced neovascularization. When formulated in a methylcellulose eye drop, VS remained intact and functional in a 4 °C solution for more than 7 days. Topical application of VS eye drops potently inhibited bFGF induced neovascularization in rat corneas. Conclusions: The present study effectively demonstrated the potential feasibility of local application of VS for treatment of corneal angiogenesis.

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Wu PC, Yang LC, Kuo HK, Huang CC, Tsai CL, Lin PR 等. Inhibition of corneal angiogenesis by local application of vasostatin. Molecular Vision. 2005 1月 13;11:28-35.