Insufficient acidification of autophagosomes facilitates group a streptococcus survival and growth in endothelial cells

Shiou Ling Lu, Chih Feng Kuo, Hao Wen Chen, Yi Shuan Yang, Ching Chuan Liu, Robert Anderson, Jiunn Jong Wu, Yee Shin Lin

研究成果: Article

16 引文 (Scopus)

摘要

Group A streptococcus (GAS) is an important human pathogen, and its invasion via blood vessels is critically important in serious events such as bacteremia or multiorgan failure. Although GAS was identified as an extracellular bacterium, the internalization of GAS into nonphagocytic cells may provide a strategy to escape from immune surveillance and antibiotic killing. However, GAS has also been reported to induce autophagy and is efficiently killed within lysosome-fused autophagosomes in epithelial cells. In this study, we show that GAS can replicate in endothelial cells and that streptolysin O is required for GAS growth. Bacterial replication can be suppressed by altering GAS gene expression in an acidic medium before internalization into endothelial cells. The inhibitory effect on GAS replication can be reversed by treatment with bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase. Compared with epithelial cells in which acidification causes autophagy-mediated clearance of GAS, there was a defect in acidification of GAS-containing vesicles in endothelial cells. Consequently, endothelial cells fail to maintain low pH in GAS-containing autophagosomes, thereby permitting GAS replication inside LAMP-1-and LC3-positive vesicles. Furthermore, treatment of epithelial cells with bafilomycin A1 resulted in defective GAS clearance by autophagy, with subsequent bacterial growth intracellularly. Therefore, low pH is a key factor for autophagy-mediated suppression of GAS growth inside epithelial cells, while defective acidification of GAS-containing vesicles results in bacterial growth in endothelial cells.

原文English
文章編號e01435-15
期刊mBio
6
發行號5
DOIs
出版狀態Published - 2015 一月 1

指紋

Streptococcus
Endothelial Cells
Survival
Growth
Autophagy
Epithelial Cells
Autophagosomes
Vacuolar Proton-Translocating ATPases
Bacteremia
Lysosomes
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology

引用此文

Lu, Shiou Ling ; Kuo, Chih Feng ; Chen, Hao Wen ; Yang, Yi Shuan ; Liu, Ching Chuan ; Anderson, Robert ; Wu, Jiunn Jong ; Lin, Yee Shin. / Insufficient acidification of autophagosomes facilitates group a streptococcus survival and growth in endothelial cells. 於: mBio. 2015 ; 卷 6, 編號 5.
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Insufficient acidification of autophagosomes facilitates group a streptococcus survival and growth in endothelial cells. / Lu, Shiou Ling; Kuo, Chih Feng; Chen, Hao Wen; Yang, Yi Shuan; Liu, Ching Chuan; Anderson, Robert; Wu, Jiunn Jong; Lin, Yee Shin.

於: mBio, 卷 6, 編號 5, e01435-15, 01.01.2015.

研究成果: Article

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AU - Kuo, Chih Feng

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AU - Liu, Ching Chuan

AU - Anderson, Robert

AU - Wu, Jiunn Jong

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