Integrated microfluidic chip for fast diagnosis of piscine nodavirus

S. H. Lee, M. C. Ou, Tzong-Yueh Chen, G. B. Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.

原文English
主出版物標題TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
頁面943-946
頁數4
DOIs
出版狀態Published - 2007 十二月 1
事件4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
持續時間: 2007 六月 102007 六月 14

出版系列

名字TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
國家France
城市Lyon
期間07-06-1007-06-14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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