Integrated microfluidic device using a single universal aptamer to detect multiple types of influenza viruses

Chih Hung Wang, Chih Peng Chang, Gwo Bin Lee

研究成果: Article

16 引文 (Scopus)

摘要

DNA aptamers that can bind specific molecular targets have great potential as probes for microbial diagnostic applications. However, aptamers may change their conformation under different operating conditions, thus affecting their affinity and specificity towards the target molecules. In this study, a new integrated microfluidic system was developed that exploited the predictable change in conformation of a single universal influenza aptamer exposed to differing ion concentrations in order to detect multiple types of the influenza virus. Furthermore, the fluorescent-labeled universal aptamer used in this system could distinguish and detect three different influenza viruses (influenza A H1N1, H3N2, and influenza B) at the same time in 20 min and therefore has great potential for point-of-care applications requiring rapid diagnosis of influenza viruses.

原文English
頁(從 - 到)247-254
頁數8
期刊Biosensors and Bioelectronics
86
DOIs
出版狀態Published - 2016 十二月 15

指紋

Lab-On-A-Chip Devices
Orthomyxoviridae
Viruses
Microfluidics
Human Influenza
Conformations
Point-of-Care Systems
Nucleotide Aptamers
Influenza A virus
DNA
Ions
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

引用此文

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