Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens

Wen Hsin Chang; Sung Yi Yang; Chih Hung Wang; Ming An Tsai; Pei Chyi Wang; Shih Chu Chen; Tzong Yueh Chen; Gwo Bin Lee

doi:10.1109/TRANSDUCERS.2011.5969305

Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Ming An Tsai, Pei Chyi Wang, Shih Chu Chen, Tzong Yueh Chen, Gwo Bin Lee

生物科技與產業科學系

研究成果: Conference contribution

3 引文斯高帕斯（Scopus）

摘要

Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore, it can carry out four LAMP reactions for multiple detection of pathogens. Successful demonstration for detection of aquaculture fish pathogens has been achieved by using the proposed microfluidic system.

原文	English
主出版物標題	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
頁面	210-213
頁數	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969305
出版狀態	Published - 2011 9月 1
事件	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China 持續時間: 2011 6月 5 → 2011 6月 9

出版系列

名字	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Other

Other	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
國家/地區	China
城市	Beijing
期間	11-06-05 → 11-06-09

All Science Journal Classification (ASJC) codes

硬體和架構
電氣與電子工程

存取文件

10.1109/TRANSDUCERS.2011.5969305

其它文件與鏈接

引用此

Chang, W. H., Yang, S. Y., Wang, C. H., Tsai, M. A., Wang, P. C., Chen, S. C., Chen, T. Y., & Lee, G. B. (2011). Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. 於 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (頁 210-213). [5969305] (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969305

Chang, Wen Hsin ; Yang, Sung Yi ; Wang, Chih Hung 等. / Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. 頁 210-213 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

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abstract = "Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore, it can carry out four LAMP reactions for multiple detection of pathogens. Successful demonstration for detection of aquaculture fish pathogens has been achieved by using the proposed microfluidic system.",

author = "Chang, {Wen Hsin} and Yang, {Sung Yi} and Wang, {Chih Hung} and Tsai, {Ming An} and Wang, {Pei Chyi} and Chen, {Shih Chu} and Chen, {Tzong Yueh} and Lee, {Gwo Bin}",

year = "2011",

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doi = "10.1109/TRANSDUCERS.2011.5969305",

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Chang, WH, Yang, SY, Wang, CH, Tsai, MA, Wang, PC, Chen, SC, Chen, TY & Lee, GB 2011, Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. 於 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969305, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, 頁 210-213, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 11-06-05. https://doi.org/10.1109/TRANSDUCERS.2011.5969305

Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. / Chang, Wen Hsin; Yang, Sung Yi; Wang, Chih Hung 等.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 210-213 5969305 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

研究成果: Conference contribution

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AU - Chang, Wen Hsin

AU - Yang, Sung Yi

AU - Wang, Chih Hung

AU - Tsai, Ming An

AU - Wang, Pei Chyi

AU - Chen, Shih Chu

AU - Chen, Tzong Yueh

AU - Lee, Gwo Bin

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AB - Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore, it can carry out four LAMP reactions for multiple detection of pathogens. Successful demonstration for detection of aquaculture fish pathogens has been achieved by using the proposed microfluidic system.

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BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

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ER -

Chang WH, Yang SY, Wang CH, Tsai MA, Wang PC, Chen SC 等. Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. 於 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 210-213. 5969305. (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). doi: 10.1109/TRANSDUCERS.2011.5969305

Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens

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