Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

Chih Hung Wang, Kang Yi Lien, Ting Yu Wang, Tzong Yueh Chen, Gwo Bin Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

原文English
主出版物標題14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
頁面812-814
頁數3
出版狀態Published - 2010 12月 1
事件14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
持續時間: 2010 10月 32010 10月 7

出版系列

名字14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
國家/地區Netherlands
城市Groningen
期間10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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