Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers

Anirban Sinha, Yi Da Chung, Priya Gopinathan, Lien Yu Hung, Ching Hsuan Yang, Shu-Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

1 引文 (Scopus)

摘要

Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.

原文English
主出版物標題20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
發行者Chemical and Biological Microsystems Society
頁面67-68
頁數2
ISBN(電子)9780979806490
出版狀態Published - 2016 一月 1
事件20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
持續時間: 2016 十月 92016 十月 13

出版系列

名字20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
國家Ireland
城市Dublin
期間16-10-0916-10-13

指紋

Biomarkers
Microfluidics
Antibodies
Screening
Ligands
Antigens
Demonstrations
Proteins
Natriuretic Peptides

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

引用此文

Sinha, A., Chung, Y. D., Gopinathan, P., Hung, L. Y., Yang, C. H., Shiesh, S-C., & Lee, G. B. (2016). Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (頁 67-68). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Sinha, Anirban ; Chung, Yi Da ; Gopinathan, Priya ; Hung, Lien Yu ; Yang, Ching Hsuan ; Shiesh, Shu-Chu ; Lee, Gwo Bin. / Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. 頁 67-68 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
@inproceedings{ce50704c31b14c05a1a7a2630b8369e0,
title = "Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers",
abstract = "Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.",
author = "Anirban Sinha and Chung, {Yi Da} and Priya Gopinathan and Hung, {Lien Yu} and Yang, {Ching Hsuan} and Shu-Chu Shiesh and Lee, {Gwo Bin}",
year = "2016",
month = "1",
day = "1",
language = "English",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "67--68",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

}

Sinha, A, Chung, YD, Gopinathan, P, Hung, LY, Yang, CH, Shiesh, S-C & Lee, GB 2016, Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, 頁 67-68, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16-10-09.

Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. / Sinha, Anirban; Chung, Yi Da; Gopinathan, Priya; Hung, Lien Yu; Yang, Ching Hsuan; Shiesh, Shu-Chu; Lee, Gwo Bin.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 67-68 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

研究成果: Conference contribution

TY - GEN

T1 - Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers

AU - Sinha, Anirban

AU - Chung, Yi Da

AU - Gopinathan, Priya

AU - Hung, Lien Yu

AU - Yang, Ching Hsuan

AU - Shiesh, Shu-Chu

AU - Lee, Gwo Bin

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.

AB - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.

UR - http://www.scopus.com/inward/record.url?scp=85014221547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014221547&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85014221547

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 67

EP - 68

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

ER -

Sinha A, Chung YD, Gopinathan P, Hung LY, Yang CH, Shiesh S-C 等. Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 67-68. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).