Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

Fu Chun Yang, Yi Lung Chen, Sen Lin Tang, Chang Ping Yu, Po Hsiang Wang, Wael Ismail, Chia Hsiang Wang, Jiun Yan Ding, Cheng Yu Yang, Chia Ying Yang, Yin Ru Chiang

研究成果: Article

18 引文 (Scopus)

摘要

Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotope-labeled substrates are not easily available.

原文English
頁(從 - 到)1967-1983
頁數17
期刊ISME Journal
10
發行號8
DOIs
出版狀態Published - 2016 八月 1

指紋

biochemical mechanisms
androgen
androgens
biodegradation
Androgens
phylogenetics
phylogeny
17-Hydroxysteroid Dehydrogenases
testosterone
steroid
Sewage
Hydro-Lyases
Steroids
sewage
degradation
steroids
Thauera
metabolite
substrate preference
metabolites

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

引用此文

Yang, Fu Chun ; Chen, Yi Lung ; Tang, Sen Lin ; Yu, Chang Ping ; Wang, Po Hsiang ; Ismail, Wael ; Wang, Chia Hsiang ; Ding, Jiun Yan ; Yang, Cheng Yu ; Yang, Chia Ying ; Chiang, Yin Ru. / Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment. 於: ISME Journal. 2016 ; 卷 10, 編號 8. 頁 1967-1983.
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abstract = "Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotope-labeled substrates are not easily available.",
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Yang, FC, Chen, YL, Tang, SL, Yu, CP, Wang, PH, Ismail, W, Wang, CH, Ding, JY, Yang, CY, Yang, CY & Chiang, YR 2016, 'Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment', ISME Journal, 卷 10, 編號 8, 頁 1967-1983. https://doi.org/10.1038/ismej.2015.255

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment. / Yang, Fu Chun; Chen, Yi Lung; Tang, Sen Lin; Yu, Chang Ping; Wang, Po Hsiang; Ismail, Wael; Wang, Chia Hsiang; Ding, Jiun Yan; Yang, Cheng Yu; Yang, Chia Ying; Chiang, Yin Ru.

於: ISME Journal, 卷 10, 編號 8, 01.08.2016, p. 1967-1983.

研究成果: Article

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AU - Ismail, Wael

AU - Wang, Chia Hsiang

AU - Ding, Jiun Yan

AU - Yang, Cheng Yu

AU - Yang, Chia Ying

AU - Chiang, Yin Ru

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AB - Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotope-labeled substrates are not easily available.

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