摘要
We report a microfluidic system for automatic mitochondrial mutation diagnostics from sample purification to quantitative analysis. The system achieved direct DNA (mtDNA) mutation quantification in affected cells using a new 3D-microfluidic system, which integrated a mtDNA extraction module and a mutation detection module. Effective direct mtDNA extraction from the cells was realized using magnetic field manipulation. The obtained mtDNAs were subject to a fully automatic processing for quantitative mutation detection using integrated micropumps, micromixer and microtemperature control modules capable of mutation sensing by restriction enzyme digestion and real-time on-chip micro-PCR. Compared with traditional methods, this microfluidic system demonstrates the advantages of faster detection, requirement of fewer amount of specimens and reagents, much compact design and lower cost as well as lower risks for human errors. Thus, such system-on-chip would encourage the future translational development of rapid pathogenic mtDNA defects detection to provide more efficient clinical diagnosis and disease management strategies.
| 原文 | English |
|---|---|
| 頁（從 - 到） | 6-11 |
| 頁數 | 6 |
| 期刊 | Biosensors and Bioelectronics |
| 卷 | 48 |
| DOIs | |
| 出版狀態 | Published - 2013 十月 5 |
指紋
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
引用此文
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Integrated three-dimensional system-on-chip for direct quantitative detection of mitochondrial DNA mutation in affected cells. / Chang, Chen Min; Chiu, Li Fang; Wei, Yau Huei; Shieh, Dar Bin; Lee, Gwo Bin.
於: Biosensors and Bioelectronics, 卷 48, 05.10.2013, p. 6-11.研究成果: Article
TY - JOUR
T1 - Integrated three-dimensional system-on-chip for direct quantitative detection of mitochondrial DNA mutation in affected cells
AU - Chang, Chen Min
AU - Chiu, Li Fang
AU - Wei, Yau Huei
AU - Shieh, Dar Bin
AU - Lee, Gwo Bin
PY - 2013/10/5
Y1 - 2013/10/5
N2 - We report a microfluidic system for automatic mitochondrial mutation diagnostics from sample purification to quantitative analysis. The system achieved direct DNA (mtDNA) mutation quantification in affected cells using a new 3D-microfluidic system, which integrated a mtDNA extraction module and a mutation detection module. Effective direct mtDNA extraction from the cells was realized using magnetic field manipulation. The obtained mtDNAs were subject to a fully automatic processing for quantitative mutation detection using integrated micropumps, micromixer and microtemperature control modules capable of mutation sensing by restriction enzyme digestion and real-time on-chip micro-PCR. Compared with traditional methods, this microfluidic system demonstrates the advantages of faster detection, requirement of fewer amount of specimens and reagents, much compact design and lower cost as well as lower risks for human errors. Thus, such system-on-chip would encourage the future translational development of rapid pathogenic mtDNA defects detection to provide more efficient clinical diagnosis and disease management strategies.
AB - We report a microfluidic system for automatic mitochondrial mutation diagnostics from sample purification to quantitative analysis. The system achieved direct DNA (mtDNA) mutation quantification in affected cells using a new 3D-microfluidic system, which integrated a mtDNA extraction module and a mutation detection module. Effective direct mtDNA extraction from the cells was realized using magnetic field manipulation. The obtained mtDNAs were subject to a fully automatic processing for quantitative mutation detection using integrated micropumps, micromixer and microtemperature control modules capable of mutation sensing by restriction enzyme digestion and real-time on-chip micro-PCR. Compared with traditional methods, this microfluidic system demonstrates the advantages of faster detection, requirement of fewer amount of specimens and reagents, much compact design and lower cost as well as lower risks for human errors. Thus, such system-on-chip would encourage the future translational development of rapid pathogenic mtDNA defects detection to provide more efficient clinical diagnosis and disease management strategies.
UR - http://www.scopus.com/inward/record.url?scp=84877067261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877067261&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2013.02.015
DO - 10.1016/j.bios.2013.02.015
M3 - Article
C2 - 23639343
AN - SCOPUS:84877067261
VL - 48
SP - 6
EP - 11
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -