Intracellular alkalinization in dexamethasone-induced thymocyte apoptosis

H. Y. Lei, M. J. Tang, N. Tsao

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)


Glucocorticoid can induce apoptosis of thymocytes, but its mechanism is not clear yet. In this study, we reported that dexamethasone-induced apoptosis was associated with intracellular alkalinization. Dexamethasone induced a higher percentage of apoptosis in 138 mM than in 50 mM NaCl, total abrogation of apoptosis was noted in NaCI-depleted culture medium. Highest apoptotic rate was observed in medium with pH 7.2, whereas it was partially and completely inhibited at pH 6.5 and pH 6.0, respectively. Intracellular pH was higher in pre-apoptotic thymocytes than non-apoptotic ones.The Na+/H+ antiporter inhibitor of 5-(N,N'-dimethyl)-amiloride inhibited the dexamethasone-induced increase in pHi and apoptosis of thymocytes. Glucocorticoid antagonist RU486 also blocked the dexamethasone-induced effect. Furthermore, the apoptosis and increase in intracellular pH induced by dexamethasone were inhibited by cycloheximide, actinomycin D. It seems that intracellular pH is increased during the development of thymocyte apoptosis and inhibiting its increment would retard the rate of progression to cell death.

頁(從 - 到)304-312
出版狀態Published - 1997

All Science Journal Classification (ASJC) codes

  • 藥理
  • 藥學科學
  • 臨床生物化學
  • 細胞生物學
  • 生物化學(醫學)
  • 癌症研究


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