Intracellular microbiome profiling of the acanthamoeba clinical isolates from lens associated keratitis

Yu Jen Wang, Sung Chou Li, Wei Chen Lin, Fu Chin Huang

研究成果: Article同行評審

摘要

Acanthamoeba act as hosts for various microorganisms and pathogens, causing Acan-thamoeba Keratitis (AK). To investigate the association between endosymbionts and AK progression, we performed a metagenomics study to characterize the intracellular microbiome from five lenses associated with AK isolates and standard strains to characterize the role of ocular flora in AK pro-gression. The used clinical isolates were axenic cultured from lenses associated with AK patients. AK isolates and standard controls such as 16S ribosomal RNA sequencing techniques were used for analysis. The microbiome compositions and relative abundance values were compared. The orders of Clostridiales and Bacteroidales presented major populations of intracellular microbes belonging to all isolates. Comparison of the different source isolates showed that most of the abundance in ker-atitis isolates came from Ruminococcus gnavus (121.0 folds), Eubacterium dolichum (54.15 folds), Rose-buria faecis (24.51 folds), and Blautia producta (3.15 folds). Further analysis of the relative abundance data from keratitis isolates showed that Blautia producta was positively correlated with the disease course. In contrast, Bacteroides ovatus was found to be abundant in early-stage keratitis isolates. This study reveals the abundant anaerobic Gram-positive rods present in severe keratitis isolate and characterize the association between Acanthamoeba and ocular flora in AK progression.

原文English
文章編號266
頁(從 - 到)1-12
頁數12
期刊Pathogens
10
發行號3
DOIs
出版狀態Published - 2021 三月

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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