Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain

C. R. Lin, Ping-Ching Wu, H. C. Shih, J. T. Cheng, C. Y. Lu, A. K. Chou, L. C. Yang

研究成果: Article

23 引文 (Scopus)

摘要

Injury to, or dysfunction of, the nervous system can lead to spontaneous pain, hyperalgesia, and/or allodynia. It is believed that the number and activity of GABAergic neurons gradually decreases over the dorsal horn. Glutamic acid decarboxylase (GAD) immunocompetence has been demonstrated on spinal progenitor cells (SPCs) cultivated in vitro. The intrathecal implantation of these cultivated progenitor cells may provide a means of alleviating neuropathic pain. Chronic constriction injury (CCI) of the sciatic nerve was used to induce chronic neuropathic pain in the hind paw of rats. SPCs (1 × 106) were implanted intrathecally on the third day after the CCI surgery. The behavioral response to thermal hyperalgesia was observed and recorded during the 14 days postsurgery. Various techniques were utilized to trace the progenitor cells, confirm the differentiation, and identify the neurotransmitters involved. GAD immunoactivity was revealed for 65% of the cultivated spinal progenitor cells in our study. We also determined that transplanted cells could survive more than 3 weeks postintrathecal implantation. Significant reductions were demonstrated for responses to thermal stimuli for the CCI rats that had received intrathecal SPC transplantation. A novel intrathecal delivery with SPCs reduced CCI-induced neuropathic pain.

原文English
頁(從 - 到)17-24
頁數8
期刊Cell Transplantation
11
發行號1
出版狀態Published - 2002 四月 3

指紋

Cell Transplantation
Neuralgia
Rats
Stem Cells
Acids
Constriction
Neurology
Ion implantation
Hyperalgesia
Surgery
Neurons
Wounds and Injuries
Glutamate Decarboxylase
Therapeutics
Immunocompetence
GABAergic Neurons
Sciatic Nerve
Hot Temperature
Chronic Pain
Nervous System

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

引用此文

Lin, C. R., Wu, P-C., Shih, H. C., Cheng, J. T., Lu, C. Y., Chou, A. K., & Yang, L. C. (2002). Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain. Cell Transplantation, 11(1), 17-24.
Lin, C. R. ; Wu, Ping-Ching ; Shih, H. C. ; Cheng, J. T. ; Lu, C. Y. ; Chou, A. K. ; Yang, L. C. / Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain. 於: Cell Transplantation. 2002 ; 卷 11, 編號 1. 頁 17-24.
@article{45c11bdc63154387a1413d2063bf3fe4,
title = "Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain",
abstract = "Injury to, or dysfunction of, the nervous system can lead to spontaneous pain, hyperalgesia, and/or allodynia. It is believed that the number and activity of GABAergic neurons gradually decreases over the dorsal horn. Glutamic acid decarboxylase (GAD) immunocompetence has been demonstrated on spinal progenitor cells (SPCs) cultivated in vitro. The intrathecal implantation of these cultivated progenitor cells may provide a means of alleviating neuropathic pain. Chronic constriction injury (CCI) of the sciatic nerve was used to induce chronic neuropathic pain in the hind paw of rats. SPCs (1 × 106) were implanted intrathecally on the third day after the CCI surgery. The behavioral response to thermal hyperalgesia was observed and recorded during the 14 days postsurgery. Various techniques were utilized to trace the progenitor cells, confirm the differentiation, and identify the neurotransmitters involved. GAD immunoactivity was revealed for 65{\%} of the cultivated spinal progenitor cells in our study. We also determined that transplanted cells could survive more than 3 weeks postintrathecal implantation. Significant reductions were demonstrated for responses to thermal stimuli for the CCI rats that had received intrathecal SPC transplantation. A novel intrathecal delivery with SPCs reduced CCI-induced neuropathic pain.",
author = "Lin, {C. R.} and Ping-Ching Wu and Shih, {H. C.} and Cheng, {J. T.} and Lu, {C. Y.} and Chou, {A. K.} and Yang, {L. C.}",
year = "2002",
month = "4",
day = "3",
language = "English",
volume = "11",
pages = "17--24",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "1",

}

Lin, CR, Wu, P-C, Shih, HC, Cheng, JT, Lu, CY, Chou, AK & Yang, LC 2002, 'Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain', Cell Transplantation, 卷 11, 編號 1, 頁 17-24.

Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain. / Lin, C. R.; Wu, Ping-Ching; Shih, H. C.; Cheng, J. T.; Lu, C. Y.; Chou, A. K.; Yang, L. C.

於: Cell Transplantation, 卷 11, 編號 1, 03.04.2002, p. 17-24.

研究成果: Article

TY - JOUR

T1 - Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain

AU - Lin, C. R.

AU - Wu, Ping-Ching

AU - Shih, H. C.

AU - Cheng, J. T.

AU - Lu, C. Y.

AU - Chou, A. K.

AU - Yang, L. C.

PY - 2002/4/3

Y1 - 2002/4/3

N2 - Injury to, or dysfunction of, the nervous system can lead to spontaneous pain, hyperalgesia, and/or allodynia. It is believed that the number and activity of GABAergic neurons gradually decreases over the dorsal horn. Glutamic acid decarboxylase (GAD) immunocompetence has been demonstrated on spinal progenitor cells (SPCs) cultivated in vitro. The intrathecal implantation of these cultivated progenitor cells may provide a means of alleviating neuropathic pain. Chronic constriction injury (CCI) of the sciatic nerve was used to induce chronic neuropathic pain in the hind paw of rats. SPCs (1 × 106) were implanted intrathecally on the third day after the CCI surgery. The behavioral response to thermal hyperalgesia was observed and recorded during the 14 days postsurgery. Various techniques were utilized to trace the progenitor cells, confirm the differentiation, and identify the neurotransmitters involved. GAD immunoactivity was revealed for 65% of the cultivated spinal progenitor cells in our study. We also determined that transplanted cells could survive more than 3 weeks postintrathecal implantation. Significant reductions were demonstrated for responses to thermal stimuli for the CCI rats that had received intrathecal SPC transplantation. A novel intrathecal delivery with SPCs reduced CCI-induced neuropathic pain.

AB - Injury to, or dysfunction of, the nervous system can lead to spontaneous pain, hyperalgesia, and/or allodynia. It is believed that the number and activity of GABAergic neurons gradually decreases over the dorsal horn. Glutamic acid decarboxylase (GAD) immunocompetence has been demonstrated on spinal progenitor cells (SPCs) cultivated in vitro. The intrathecal implantation of these cultivated progenitor cells may provide a means of alleviating neuropathic pain. Chronic constriction injury (CCI) of the sciatic nerve was used to induce chronic neuropathic pain in the hind paw of rats. SPCs (1 × 106) were implanted intrathecally on the third day after the CCI surgery. The behavioral response to thermal hyperalgesia was observed and recorded during the 14 days postsurgery. Various techniques were utilized to trace the progenitor cells, confirm the differentiation, and identify the neurotransmitters involved. GAD immunoactivity was revealed for 65% of the cultivated spinal progenitor cells in our study. We also determined that transplanted cells could survive more than 3 weeks postintrathecal implantation. Significant reductions were demonstrated for responses to thermal stimuli for the CCI rats that had received intrathecal SPC transplantation. A novel intrathecal delivery with SPCs reduced CCI-induced neuropathic pain.

UR - http://www.scopus.com/inward/record.url?scp=0036127021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036127021&partnerID=8YFLogxK

M3 - Article

C2 - 12095216

AN - SCOPUS:0036127021

VL - 11

SP - 17

EP - 24

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 1

ER -