Investigation of lipases from various Carica papaya varieties for hydrolysis of olive oil and kinetic resolution of (R,S)-profen 2,2,2-trifluoroethyl thioesters

I. Son Ng, Shau Wei Tsai

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

With olive oil hydrolysis in aqueous solutions and hydrolytic resolution of (R,S)-profen 2,2,2-trifluoroethyl thioesters in water-saturated isooctane as the model systems, the lipolysis and enantioselective hydrolysis activities of four partially purified Carica papaya lipases of different plant variety and geography location of cultures were compared to select pCPL-Indo from Indonesia as the best lipase preparation. For lipolysis, an optimal pH of 8.5 for all lipase preparations was found. Yet, pCPL-Indo possessed the highest activity at pH ranged from 7 to 10. For the kinetic resolution, the thermodynamic analysis implied that pCPL-Indo has changed the conformation at 60°C and the enantiomer discrimination was dominated by ΔΔH. The kinetic analysis also indicated that the enantiomeric discrimination was mainly due to the difference of k2S and k2R in the acylation step. Agreements between experimental time-course conversions XS and best-fitted results were illustrated by considering effects of product inhibition and enzyme deactivation.

原文English
頁(從 - 到)540-546
頁數7
期刊Process Biochemistry
41
發行號3
DOIs
出版狀態Published - 2006 3月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 應用微生物與生物技術

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