Ion concentration polarization on paper-based microfluidic devices and its application to preconcentrate dilute sample solutions

Ruey Jen Yang, Hao Hsuan Pu, Hsiang Li Wang

研究成果: Article

45 引文 斯高帕斯(Scopus)

摘要

Microfluidic paper-based analytical devices (?PADs) are a promising solution for a wide range of point-of-care applications. The feasibility of inducing ion concentration polarization (ICP) on ?PADs has thus far attracted little attention. Accordingly, this study commences by demonstrating the ICP phenomenon in a ?PAD with a Nafion ion-selective membrane. We are the first to measure the current-voltage curve on a Nafion-coated ?PAD in order to indicate that the ion depletion occurs and the ICP is triggered when the current reaches the limiting current. The ICP effect is then exploited to preconcentrate fluorescein on ?PADs incorporating straight and convergent channels. By an optimal geometric design, it is shown that the convergent channel results in a greater preconcentration effect than the straight channel. Specifically, a 20-fold enhancement in the sample concentration is achieved after 130?s given an initial concentration of 10?5 M and an external potential of 50?V. By contrast, the straight channel yields only a 10-fold improvement in the concentration after 180?s. Further, the practical feasibility of the proposed convergent-channel ?PAD is demonstrated using fluorescein isothiocyanate labeled bovine serum albumin. The experimental results show that a 15-fold enhancement of the initial sample concentration (10?5 M) is obtained after 120?s given an external potential of 50?V.

原文English
文章編號014122
期刊Biomicrofluidics
9
發行號1
DOIs
出版狀態Published - 2015 二月 18

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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