Isobolographic analysis of the cutaneous antinociceptive interaction between bupivacaine co-injected with serotonin in rats

Jann Inn Tzeng, Chong Chi Chiu, Jhi Joung Wang, Yu Wen Chen, Ching-Hsia Hung

研究成果: Article

8 引文 (Scopus)

摘要

Background The aim of this experiment was to investigate a long-lasting local anesthetic bupivacaine combined with serotonin at inducing cutaneous antinociception. Methods The skin antinociception, characterized by an inhibition of the cutaneous trunci muscle reflex (CTMR) following the pinprick on the dorsal skin of rats, was evaluated. The cutaneous antinociceptive effects of bupivacaine alone, serotonin alone, or bupivacaine co-injected with serotonin in a dose-dependent fashion were constructed, while the drug–drug interactions were evaluated by isobologram. Results Subcutaneous serotonin, as well as the local anesthetic bupivacaine provoked dose-related cutaneous antinociception. On an equipotent basis (50% effective dose [ED 50 ]), the relative potency was bupivacaine (0.43 [0.37–0.50] μmol) > serotonin (1.27 [1.15–1.40] μmol) (p < 0.01). At the equi-anesthetic doses (ED 75 , ED 50 and ED 25 ), the duration of bupivacaine was similar to that of serotonin at producing cutaneous antinociceptive effects. Co-administration of bupivacaine and serotonin displayed a synergistic antinociception. Conclusions The preclinical data demonstrated that serotonin is less potent in eliciting cutaneous antinociceptive effects but has the similar duration of action, compared with bupivacaine. We also found a more significant depth of the sensory block with bupivacaine + serotonin than bupivacaine alone.

原文English
頁(從 - 到)846-850
頁數5
期刊Pharmacological Reports
69
發行號5
DOIs
出版狀態Published - 2017 十月 1

指紋

Bupivacaine
Serotonin
Skin
Local Anesthetics
Reflex
Anesthetics
Muscles

All Science Journal Classification (ASJC) codes

  • Pharmacology

引用此文

Tzeng, Jann Inn ; Chiu, Chong Chi ; Wang, Jhi Joung ; Chen, Yu Wen ; Hung, Ching-Hsia. / Isobolographic analysis of the cutaneous antinociceptive interaction between bupivacaine co-injected with serotonin in rats. 於: Pharmacological Reports. 2017 ; 卷 69, 編號 5. 頁 846-850.
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abstract = "Background The aim of this experiment was to investigate a long-lasting local anesthetic bupivacaine combined with serotonin at inducing cutaneous antinociception. Methods The skin antinociception, characterized by an inhibition of the cutaneous trunci muscle reflex (CTMR) following the pinprick on the dorsal skin of rats, was evaluated. The cutaneous antinociceptive effects of bupivacaine alone, serotonin alone, or bupivacaine co-injected with serotonin in a dose-dependent fashion were constructed, while the drug–drug interactions were evaluated by isobologram. Results Subcutaneous serotonin, as well as the local anesthetic bupivacaine provoked dose-related cutaneous antinociception. On an equipotent basis (50{\%} effective dose [ED 50 ]), the relative potency was bupivacaine (0.43 [0.37–0.50] μmol) > serotonin (1.27 [1.15–1.40] μmol) (p < 0.01). At the equi-anesthetic doses (ED 75 , ED 50 and ED 25 ), the duration of bupivacaine was similar to that of serotonin at producing cutaneous antinociceptive effects. Co-administration of bupivacaine and serotonin displayed a synergistic antinociception. Conclusions The preclinical data demonstrated that serotonin is less potent in eliciting cutaneous antinociceptive effects but has the similar duration of action, compared with bupivacaine. We also found a more significant depth of the sensory block with bupivacaine + serotonin than bupivacaine alone.",
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Isobolographic analysis of the cutaneous antinociceptive interaction between bupivacaine co-injected with serotonin in rats. / Tzeng, Jann Inn; Chiu, Chong Chi; Wang, Jhi Joung; Chen, Yu Wen; Hung, Ching-Hsia.

於: Pharmacological Reports, 卷 69, 編號 5, 01.10.2017, p. 846-850.

研究成果: Article

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AU - Tzeng, Jann Inn

AU - Chiu, Chong Chi

AU - Wang, Jhi Joung

AU - Chen, Yu Wen

AU - Hung, Ching-Hsia

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N2 - Background The aim of this experiment was to investigate a long-lasting local anesthetic bupivacaine combined with serotonin at inducing cutaneous antinociception. Methods The skin antinociception, characterized by an inhibition of the cutaneous trunci muscle reflex (CTMR) following the pinprick on the dorsal skin of rats, was evaluated. The cutaneous antinociceptive effects of bupivacaine alone, serotonin alone, or bupivacaine co-injected with serotonin in a dose-dependent fashion were constructed, while the drug–drug interactions were evaluated by isobologram. Results Subcutaneous serotonin, as well as the local anesthetic bupivacaine provoked dose-related cutaneous antinociception. On an equipotent basis (50% effective dose [ED 50 ]), the relative potency was bupivacaine (0.43 [0.37–0.50] μmol) > serotonin (1.27 [1.15–1.40] μmol) (p < 0.01). At the equi-anesthetic doses (ED 75 , ED 50 and ED 25 ), the duration of bupivacaine was similar to that of serotonin at producing cutaneous antinociceptive effects. Co-administration of bupivacaine and serotonin displayed a synergistic antinociception. Conclusions The preclinical data demonstrated that serotonin is less potent in eliciting cutaneous antinociceptive effects but has the similar duration of action, compared with bupivacaine. We also found a more significant depth of the sensory block with bupivacaine + serotonin than bupivacaine alone.

AB - Background The aim of this experiment was to investigate a long-lasting local anesthetic bupivacaine combined with serotonin at inducing cutaneous antinociception. Methods The skin antinociception, characterized by an inhibition of the cutaneous trunci muscle reflex (CTMR) following the pinprick on the dorsal skin of rats, was evaluated. The cutaneous antinociceptive effects of bupivacaine alone, serotonin alone, or bupivacaine co-injected with serotonin in a dose-dependent fashion were constructed, while the drug–drug interactions were evaluated by isobologram. Results Subcutaneous serotonin, as well as the local anesthetic bupivacaine provoked dose-related cutaneous antinociception. On an equipotent basis (50% effective dose [ED 50 ]), the relative potency was bupivacaine (0.43 [0.37–0.50] μmol) > serotonin (1.27 [1.15–1.40] μmol) (p < 0.01). At the equi-anesthetic doses (ED 75 , ED 50 and ED 25 ), the duration of bupivacaine was similar to that of serotonin at producing cutaneous antinociceptive effects. Co-administration of bupivacaine and serotonin displayed a synergistic antinociception. Conclusions The preclinical data demonstrated that serotonin is less potent in eliciting cutaneous antinociceptive effects but has the similar duration of action, compared with bupivacaine. We also found a more significant depth of the sensory block with bupivacaine + serotonin than bupivacaine alone.

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