TY - JOUR
T1 - Isolating stromal stem cells from periodontal granulation tissues
AU - Hung, Tzu Yuan
AU - Lin, Hsiang Chun
AU - Chan, Ying Jen
AU - Yuan, Kuo
N1 - Funding Information:
Acknowledgements This study was supported in part by the National Science Council, Taiwan under grant NSC 96–2314-B-006– 069. We would like to thank two anonymous (unknown) reviewers and the editor for their comments.
PY - 2012/8
Y1 - 2012/8
N2 - Stem cell therapy is a promising area in regenerative medicine. Periodontal granulation tissues are often discarded during conventional surgery. If stromal stem cells can be isolated from these tissues, they can be used for subsequent surgery on the same patient. Fifteen human periodontal granulation tissue samples were obtained from intrabony defects during surgery. Immunohistochemistry (IHC) was carried out on five of the samples to identify STRO-1, a marker of mesenchymal stem cells. Five samples underwent flow cytometry analysis for the same marker. The remaining five samples were characterized by "colony formation unit-fibroblast" (CFU-f) assay and selected for proliferation assay, flow cytometry of stem cell markers, immunocytochemistry (ICC), multipotent differentiation assays, and repairing critical-size defects in mice. The ratio of STRO-1+ cells detected by IHC was 5. 91 ± 1. 50%. The analysis of flow cytometry for STRO-1 was 6. 70 ± 0. 81%. Approximately two thirds of the CFU-f colonies had a strong reaction to STRO-1 in ICC staining. The cells were multipotent both in vitro and in vivo. Mice given bone grafts and stem cells showed significantly better bone healing than those without stem cells. Multipotent stromal stem cells can be isolated from human periodontal granulation tissues. These cells improve new bone formation when transplanted in mouse calvarial defects. Isolating stem cells from relatively accessible sites without extra procedures is clinically advantageous. This study demonstrated that human periodontal granulation tissues contain isolatable multipotent stem cells. The cells may be a good source for autotransplantation in subsequent treatment.
AB - Stem cell therapy is a promising area in regenerative medicine. Periodontal granulation tissues are often discarded during conventional surgery. If stromal stem cells can be isolated from these tissues, they can be used for subsequent surgery on the same patient. Fifteen human periodontal granulation tissue samples were obtained from intrabony defects during surgery. Immunohistochemistry (IHC) was carried out on five of the samples to identify STRO-1, a marker of mesenchymal stem cells. Five samples underwent flow cytometry analysis for the same marker. The remaining five samples were characterized by "colony formation unit-fibroblast" (CFU-f) assay and selected for proliferation assay, flow cytometry of stem cell markers, immunocytochemistry (ICC), multipotent differentiation assays, and repairing critical-size defects in mice. The ratio of STRO-1+ cells detected by IHC was 5. 91 ± 1. 50%. The analysis of flow cytometry for STRO-1 was 6. 70 ± 0. 81%. Approximately two thirds of the CFU-f colonies had a strong reaction to STRO-1 in ICC staining. The cells were multipotent both in vitro and in vivo. Mice given bone grafts and stem cells showed significantly better bone healing than those without stem cells. Multipotent stromal stem cells can be isolated from human periodontal granulation tissues. These cells improve new bone formation when transplanted in mouse calvarial defects. Isolating stem cells from relatively accessible sites without extra procedures is clinically advantageous. This study demonstrated that human periodontal granulation tissues contain isolatable multipotent stem cells. The cells may be a good source for autotransplantation in subsequent treatment.
UR - http://www.scopus.com/inward/record.url?scp=84864289024&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864289024&partnerID=8YFLogxK
U2 - 10.1007/s00784-011-0600-5
DO - 10.1007/s00784-011-0600-5
M3 - Article
C2 - 21837388
AN - SCOPUS:84864289024
SN - 1432-6981
VL - 16
SP - 1171
EP - 1180
JO - Clinical Oral Investigations
JF - Clinical Oral Investigations
IS - 4
ER -
