Isolation and characterization of mammalian HDAC10, a novel histone deacetylase

Hung Ying Kao, Chih Hao Lee, Andrei Komarov, Chris C. Han, Ronald M. Evans

研究成果: Article同行評審

150 引文 斯高帕斯(Scopus)

摘要

Acetylation of histone core particles plays an important role in modulating chromatin structure and gene expression. The acetylation status of the histone tails is determined by two opposing enzymatic activities, histone acetyltransferases and histone deacetylases (HDACs). Here we describe the isolation and characterization of HDAC10, a novel class II histone deacetylase. Molecular cloning and Northern blot analyses reveal that the HDAC10 transcript is widely expressed and subjected to alternative splicing. HDAC10 is both nuclear and cytoplasmic, a feature reminiscent of HDACs 4, 5, and 7. Distinct from other family members, HDAC10 harbors an amino-terminal catalytic domain and a carboxyl pseudo-repeat that shares significant homology with its catalytic domain. Mutational analysis reveals that transcriptional repression by HDAC10 requires its intrinsic histone deacetylase activity. Taken together, HDAC10 represents a distinct HDAC that may play a role in transcription regulation.

原文English
頁(從 - 到)187-193
頁數7
期刊Journal of Biological Chemistry
277
發行號1
DOIs
出版狀態Published - 2002 一月 4

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學
  • 細胞生物學

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