Isolation and characterization of microsatellite loci in Pedicularis verticillata L. using PCR-based isolation of microsatellite arrays (PIMA)

Tsurng Juhn Huang, Yi Yen Chen, Yen Ping Li, Cheng Yu Hung, Tzen-Yuh Chiang, Chang Hung Chou

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (HE) and observed (H0) heterozygosity was 0.62609-0.89662 and 0-0.95652, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure, which, in turn, can provide information for establishing conservation strategy for these endangered species.

原文English
頁(從 - 到)1389-1391
頁數3
期刊Conservation Genetics
9
發行號5
DOIs
出版狀態Published - 2008 十月 1

All Science Journal Classification (ASJC) codes

  • 生態學、進化論、行為學與系統學
  • 遺傳學

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