Isolation and characterization of polymorphic microsatellite DNA makers for Euphrasia nankotaizanensis (Orobanchaceae) and cross amplification in another Euphrasia L.

Kuo Hsiung Wang, Ming Jou Wu, Tzen Yuh Chiang, Chang Hung Chou

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

Euphrasia nankotaizanensis is an endangered flowering plant distributed restrictedly on rocky slope of high mountain peaks in central and northern Taiwan. In order to undertake a conservation program, especially given impacts of the global warming, it is essential to evaluate its genetic diversity and population structure. We described nine novel microsatellite primer pairs in E. nankotaizanensis and also examined its relative E. transmorrisonensis. The number of alleles per locus ranged from 7 to 29. Expected (H E) and observed (H O) heterozygosities ranged from 0.83 to 0.98 and 0.00 to 0.95, respectively. Eight of the nine microsatellite loci displayed significant departures from Hardy-Weinberg expectations, likely due to the loss of habitats and the small population size. Significant linkage disequilibrium (LD) was detected in most loci. Cross-species amplification of microsatellites took place at five loci. These primers may provide a tool for understanding the demography and population structure of Euphrasia species in Taiwan.

原文English
頁(從 - 到)1163-1165
頁數3
期刊Conservation Genetics
10
發行號4
DOIs
出版狀態Published - 2009 八月

All Science Journal Classification (ASJC) codes

  • 生態學、進化論、行為學與系統學
  • 遺傳學

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