Isolation and characterization of polymorphic microsatellite loci in Candidia barbata (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Tzen Yuh Chiang, Yu Min Ju, Lee Shing Fang, Chung Jian Lin

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

Candidia barbata is an endemic cyprinid fish in Taiwan and suffers habitat destruction caused by human overexploitation. In this study, a total of 14 polymorphic microsatellite loci from C. barbata were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 30 specimens of Taiwan. The mean number of alleles across loci was 4.92 ± 1.44. The levels of expected and observed heterozygosities varied from 0.1266 to 0.5079, and from 0.0667 to 0.9667, respectively. Frequencies of null alleles of the 14 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population.

原文English
頁(從 - 到)503-505
頁數3
期刊Conservation Genetics
10
發行號3
DOIs
出版狀態Published - 2009 六月

All Science Journal Classification (ASJC) codes

  • 生態學、進化論、行為學與系統學
  • 遺傳學

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