Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Chung Jian Lin, Jiang Ping Wang, Hung Du Lin, Tzen-Yuh Chiang

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

A total of 10 polymorphic microsatellite loci from Hemibarbus labeo were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 24 specimens of mainland China. The mean number of alleles across loci was 3.10 ± 1.10 and the level of expected heterozygosity varied from 0.0417 to 0.7482. Frequencies of null alleles of the 10 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population. Five primer pairs cross-amplify the microsatellites in other species, indicating transportability of the markers within the family Cyprinidae.

原文English
頁(從 - 到)788-790
頁數3
期刊Molecular Ecology Notes
7
發行號5
DOIs
出版狀態Published - 2007 九月 1

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 生態學
  • 生物化學、遺傳與分子生物學 (全部)

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