Isolation and characterization of polymorphic microsatellite loci from metapenaeopsis barbata using PCR-based isolation of microsatellite arrays (PIMA)

Tzen Yuh Chiang, Tzong Der Tzeng, Hung Du Lin, Ching Ju Cho, Feng Jiau Lin

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Over fishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as observed and expected heterozygosities in populations, ranging from 0.048 to 0.538, and 0.048 and 0.654, respectively, were detected. No deviation from Hardy-Weinberg expectations was detected at either locus. No significant linkage disequilibrium was detected in locus pairs. The polymorphic microsatellite loci will be useful for investigations of the genetic variation, population structure, and conservation genetics of this species.

原文English
頁(從 - 到)2763-2768
頁數6
期刊International journal of molecular sciences
13
發行號3
DOIs
出版狀態Published - 2012 三月

All Science Journal Classification (ASJC) codes

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

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