Isolation and characterization of two novel colon cancer cell lines from Chinese patients

Dan Ning Hu, Shiaw Min Hwang, Xi Zhang Lin, Pei Yuh Yang, Chi Hong Tsai, Qiang Huang, Hsin Yi Huang, Min Huo Hwang

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Incidence of colon cancer has increased rapidly in China. Although many colon cancer cell lines have been established previously, most of them were derived from patients from western countries. Epidemiological, clinical, cytogenetic, and molecular biological studies showed that there are considerable differences between Chinese and western countries colon cancer patients. Therefore, establishment of novel colon cancer cell line from Chinese is useful for studying the racial difference of this disease and can be important for studying the pathogenesis of colon cancer in China. In our laboratory, two novel continuous human colon cancer cell lines, SHT-1 and SHH-1, have been established in vitro from Chinese patients, and both cell lines have been passaged for 4 yr, and they have been continuously subcultured with more than 800 population doubling and without signs of senescence. Both cell lines were obtained from primary tumor tissues during colon cancer surgery. Cells grew rapidly with a doubling time of 36-39 h and a plating efficiency of 26-28%. These cells exhibited an epithelial morphology and expressed cytokeratin. Tumor developed in severe combined immunodeficient (SCID) mice 4-6 wk after inoculated subcutaneously with the cultured cancer cells. Karyotypic analysis and comparative genomic hybridization (CGH) analysis in SHT-1 cells revealed a hypertriploid modal number of 76 with numerous numerical and structural abnormalities previously linked to colon cancer. In another cell line (SHH-1), CGH analysis revealed that -1p13 was the only cytogenetic anomaly.

原文English
頁(從 - 到)105-108
頁數4
期刊In Vitro Cellular and Developmental Biology - Animal
43
發行號3-4
DOIs
出版狀態Published - 2007 四月

All Science Journal Classification (ASJC) codes

  • 發展生物學
  • 細胞生物學

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