Isolation and identification of xenobiotic aryl hydrocarbon receptor ligands in dyeing wastewater

Pei Hsin Chou, Saburo Matsui, Kentaro Misaki, Tomonari Matsuda

研究成果: Article同行評審

50 引文 斯高帕斯(Scopus)


Dyeing wastewater collected in Kyoto city, Japan, was investigated for the occurrence of aryl hydrocarbon receptor (AhR) ligands by using an AhR-responsive reporter gene assay. Concentrated extracts of wastewater samples elicited a dose-dependent increase in AhR ligand activity, and several hydrophobic HPLC fractions of the extracts were highly effective in inducing AhR ligand activity. Three potential AhR ligands were isolated from these fractions and identified to be Disperse Red 92, Disperse Yellow 64, and 3′-hydroxybenzo[b] quinophthalone by using HPLC and LC-MS/MS. Disperse Red 92, which has also been detected in the treated effluent from a sewage plant receiving the wastewater, is an anthraquinone disperse dye showing weak AhR binding affinity in the assay. Disperse Yellow 64 and 3′-hydroxybenzo[b]quinophthalone are quinoline disperse dyes capable of activating the AhR at nanomolar concentrations. In particular, Disperse Yellow 64 is a highly potent AhR ligand that was 3 times more effective in inducing AhR ligand activity than β-naphthoflavone in the assay. Quinoline disperse dyes are suggested to be a new class of xenobiotic AhR ligands which pose a danger to aquatic biota and human health.

頁(從 - 到)652-657
期刊Environmental Science and Technology
出版狀態Published - 2007 1月 15

All Science Journal Classification (ASJC) codes

  • 一般化學
  • 環境化學


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