Kindlin-1 Regulates Epidermal Growth Factor Receptor Signaling

Magdalene Michael, Rumena Begum, Grace K. Chan, Austin J. Whitewood, Daniel R. Matthews, Benjamin T. Goult, John A. McGrath, Maddy Parsons

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

Kindler syndrome is an autosomal recessive genodermatosis that results from mutations in the FERMT1 gene encoding t kindlin-1. Kindlin-1 localizes to focal adhesion and is known to contribute to the activation of integrin receptors. Most cases of Kindler syndrome show a reduction or complete absence of kindlin-1 in keratinocytes, resulting in defective integrin activation, cell adhesion, and migration. However, roles for kindlin-1 beyond integrin activation remain poorly defined. In this study we show that skin and keratinocytes from Kindler syndrome patients have significantly reduced expression levels of the EGFR, resulting in defective EGF-dependent signaling and cell migration. Mechanistically, we show that kindlin-1 can associate directly with EGFR in vitro and in keratinocytes in an EGF-dependent, integrin-independent manner and that formation of this complex is required for EGF-dependent migration. We further show that kindlin-1 acts to protect EGFR from lysosomal-mediated degradation. This shows a new role for kindlin-1 that has implications for understanding Kindler syndrome disease pathology.

原文English
頁(從 - 到)369-379
頁數11
期刊Journal of Investigative Dermatology
139
發行號2
DOIs
出版狀態Published - 2019 2月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學
  • 皮膚科
  • 細胞生物學

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