Kinetic Studies on Oxidation of Allyl Alcohol by Yeast Alcohol Dehydrogenase

T. C. Huang, F. Y. Ke, D. H. Chen

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The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.

頁(從 - 到)171-182
期刊Chemical Engineering Communications
出版狀態Published - 1995 五月 1

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 化學工程 (全部)


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