The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.
All Science Journal Classification (ASJC) codes
- 化學 (全部)
- 化學工程 (全部)