TY - JOUR
T1 - Kinetic study on substrate and product inhibitions for the formation of 7-amino-3-deacetoxy cephalosporanic acid from cephalosporin G by immobilized penicillin G acylase
AU - Pan, Jian Lian
AU - Syu, Mei Jywan
PY - 2005/5/1
Y1 - 2005/5/1
N2 - Penicillin G acylase (PGA) is an important enzyme in β-lactam antibiotics pharmaceutical industry for the production of 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) from cephalosporin G (Ceph-G) as well as 6-aminopenicillanic acid (6-APA) from penicillin G (Pen-G). In this work, immobilized PGA was utilized to catalyze the conversion of Ceph-G to 7-ADCA. Higher concentration of Ceph-G was found to show inhibition effect on the reaction. Both 7-ADCA and PAA (phenylacetic acid) were found to inhibit the activity of PGA, however, via different mechanisms. In this work, with low concentrations of Ceph-G substrate, the inhibited kinetic models were obtained by adding different concentrations of PAA or 7-ADCA. The influence from both products was discussed and compared. Both inhibition kinetic mechanisms were different in that PAA showed noncompetitive inhibition to PGA whereas 7-ADCA appeared to be of competitive inhibition.
AB - Penicillin G acylase (PGA) is an important enzyme in β-lactam antibiotics pharmaceutical industry for the production of 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) from cephalosporin G (Ceph-G) as well as 6-aminopenicillanic acid (6-APA) from penicillin G (Pen-G). In this work, immobilized PGA was utilized to catalyze the conversion of Ceph-G to 7-ADCA. Higher concentration of Ceph-G was found to show inhibition effect on the reaction. Both 7-ADCA and PAA (phenylacetic acid) were found to inhibit the activity of PGA, however, via different mechanisms. In this work, with low concentrations of Ceph-G substrate, the inhibited kinetic models were obtained by adding different concentrations of PAA or 7-ADCA. The influence from both products was discussed and compared. Both inhibition kinetic mechanisms were different in that PAA showed noncompetitive inhibition to PGA whereas 7-ADCA appeared to be of competitive inhibition.
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U2 - 10.1016/j.bej.2005.01.017
DO - 10.1016/j.bej.2005.01.017
M3 - Article
AN - SCOPUS:25644453537
SN - 1369-703X
VL - 23
SP - 203
EP - 210
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 3
ER -