Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral

De Wei Chang, Meng Ling Hsieh, Yan Min Chen, Tsair Fuh Lin, Jo Shu Chang

研究成果: Article同行評審

49 引文 斯高帕斯(Scopus)

摘要

The effect of an algal metabolite, β-cyclocitral, on the cell integrity of two cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed to various concentrations of β-cyclocitral. Scanning electron microscope (SEM) results indicate that the cells of tested species were greatly altered after being exposed to β-cyclocitral. A flow cytometer coupled with the SYTOX stain and chlorophyll-a auto-fluorescence was used to quantify the effect of β-cyclocitral on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments show that about 5-10mgL-1 of β-cyclocitral for the two M. aeruginosa strains and a much lower concentration, 0.1-0.5mgL-1, for N. palea were needed to cause 15-20% of cells to rupture. When the β-cyclocitral concentration was increased to 200-1000mgL-1 for M. aeruginosa and 5-10mgL-1 for N. palea, almost all the cells ruptured between 8 and 24h. A first-order kinetic model is able to describe the data of cell integrity over time. The extracted rate constant values well correlate with the applied β-cyclocitral dosages. The obtained kinetic parameters may be used to estimate β-cyclocitral dosage and contact time required for the control of cyanobacteria and diatoms in water bodies.

原文English
頁(從 - 到)1214-1220
頁數7
期刊Journal of Hazardous Materials
185
發行號2-3
DOIs
出版狀態Published - 2011 1月 30

All Science Journal Classification (ASJC) codes

  • 污染
  • 廢物管理和處置
  • 健康、毒理學和誘變
  • 環境工程
  • 環境化學

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