Krüpple-like factor 10 regulates radio-sensitivity of pancreatic cancer via UV radiation resistance-associated gene

Vincent Hung Shu Chang, Yi Chih Tsai, Ya Li Tsai, Shu-Ling Peng, Su Liang Chen, Tsung Ming Chang, Winston Chun Yuan Yu, Hui Ju Ch'ang

研究成果: Article

3 引文 (Scopus)

摘要

Background and purpose Krüpple-like factor 10 (Klf10), an early response gene of TGFβ, was reported to be a prognostic biomarker for pancreatic cancer survival. The role of Klf10 in predicting tumor response to cancer treatment is unknown. Materials and methods Genetically manipulated MiaPaCa and Panc-1 cells were established to evaluate clonogenic survival, autophagy, apoptosis and DNA repair after radiation. The interaction between Klf10 and UV radiation resistance-associated gene (UVRAG) was demonstrated by ChiP-PCR and luciferase reporter assay. Orthotopic murine tumor model and clinical specimens were used to evaluate radio-sensitivity of pancreatic cancer. Results We found Klf10 silencing correlates with enhanced pancreatic cancer clonogenic survival and murine tumor growth after radiation. UVRAG was an essential down-stream mediator transcriptionally suppressed by Klf10. Silencing UVRAG mRNA in Klf10 depleted Panc-1 cells reversed the radio-resistant phenotypes including decreased apoptosis and enhanced DNA repair as well as autophagy. Metformin, an anti-diabetic agent, was found to increase Klf10 and suppress UVRAG expression to improve radiation cytotoxicity in pancreatic cancer. The predictive value of Klf10 in radiation response and the inverse correlation with UVRAG were confirmed in cohorts of pancreatic cancer patients. Conclusions Klf10 is a potential biomarker in predicting and sensitizing radiation effect in pancreatic cancer.

原文English
頁(從 - 到)476-484
頁數9
期刊Radiotherapy and Oncology
122
發行號3
DOIs
出版狀態Published - 2017 三月 1

指紋

Radio
Pancreatic Neoplasms
Radiation
Genes
Autophagy
DNA Repair
Survival
Neoplasms
Biomarkers
Apoptosis
Metformin
Essential Genes
Radiation Effects
Luciferases
Phenotype
Gene Expression
Polymerase Chain Reaction
Messenger RNA
Growth

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging

引用此文

Chang, Vincent Hung Shu ; Tsai, Yi Chih ; Tsai, Ya Li ; Peng, Shu-Ling ; Chen, Su Liang ; Chang, Tsung Ming ; Yu, Winston Chun Yuan ; Ch'ang, Hui Ju. / Krüpple-like factor 10 regulates radio-sensitivity of pancreatic cancer via UV radiation resistance-associated gene. 於: Radiotherapy and Oncology. 2017 ; 卷 122, 編號 3. 頁 476-484.
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abstract = "Background and purpose Kr{\"u}pple-like factor 10 (Klf10), an early response gene of TGFβ, was reported to be a prognostic biomarker for pancreatic cancer survival. The role of Klf10 in predicting tumor response to cancer treatment is unknown. Materials and methods Genetically manipulated MiaPaCa and Panc-1 cells were established to evaluate clonogenic survival, autophagy, apoptosis and DNA repair after radiation. The interaction between Klf10 and UV radiation resistance-associated gene (UVRAG) was demonstrated by ChiP-PCR and luciferase reporter assay. Orthotopic murine tumor model and clinical specimens were used to evaluate radio-sensitivity of pancreatic cancer. Results We found Klf10 silencing correlates with enhanced pancreatic cancer clonogenic survival and murine tumor growth after radiation. UVRAG was an essential down-stream mediator transcriptionally suppressed by Klf10. Silencing UVRAG mRNA in Klf10 depleted Panc-1 cells reversed the radio-resistant phenotypes including decreased apoptosis and enhanced DNA repair as well as autophagy. Metformin, an anti-diabetic agent, was found to increase Klf10 and suppress UVRAG expression to improve radiation cytotoxicity in pancreatic cancer. The predictive value of Klf10 in radiation response and the inverse correlation with UVRAG were confirmed in cohorts of pancreatic cancer patients. Conclusions Klf10 is a potential biomarker in predicting and sensitizing radiation effect in pancreatic cancer.",
author = "Chang, {Vincent Hung Shu} and Tsai, {Yi Chih} and Tsai, {Ya Li} and Shu-Ling Peng and Chen, {Su Liang} and Chang, {Tsung Ming} and Yu, {Winston Chun Yuan} and Ch'ang, {Hui Ju}",
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Krüpple-like factor 10 regulates radio-sensitivity of pancreatic cancer via UV radiation resistance-associated gene. / Chang, Vincent Hung Shu; Tsai, Yi Chih; Tsai, Ya Li; Peng, Shu-Ling; Chen, Su Liang; Chang, Tsung Ming; Yu, Winston Chun Yuan; Ch'ang, Hui Ju.

於: Radiotherapy and Oncology, 卷 122, 編號 3, 01.03.2017, p. 476-484.

研究成果: Article

TY - JOUR

T1 - Krüpple-like factor 10 regulates radio-sensitivity of pancreatic cancer via UV radiation resistance-associated gene

AU - Chang, Vincent Hung Shu

AU - Tsai, Yi Chih

AU - Tsai, Ya Li

AU - Peng, Shu-Ling

AU - Chen, Su Liang

AU - Chang, Tsung Ming

AU - Yu, Winston Chun Yuan

AU - Ch'ang, Hui Ju

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Background and purpose Krüpple-like factor 10 (Klf10), an early response gene of TGFβ, was reported to be a prognostic biomarker for pancreatic cancer survival. The role of Klf10 in predicting tumor response to cancer treatment is unknown. Materials and methods Genetically manipulated MiaPaCa and Panc-1 cells were established to evaluate clonogenic survival, autophagy, apoptosis and DNA repair after radiation. The interaction between Klf10 and UV radiation resistance-associated gene (UVRAG) was demonstrated by ChiP-PCR and luciferase reporter assay. Orthotopic murine tumor model and clinical specimens were used to evaluate radio-sensitivity of pancreatic cancer. Results We found Klf10 silencing correlates with enhanced pancreatic cancer clonogenic survival and murine tumor growth after radiation. UVRAG was an essential down-stream mediator transcriptionally suppressed by Klf10. Silencing UVRAG mRNA in Klf10 depleted Panc-1 cells reversed the radio-resistant phenotypes including decreased apoptosis and enhanced DNA repair as well as autophagy. Metformin, an anti-diabetic agent, was found to increase Klf10 and suppress UVRAG expression to improve radiation cytotoxicity in pancreatic cancer. The predictive value of Klf10 in radiation response and the inverse correlation with UVRAG were confirmed in cohorts of pancreatic cancer patients. Conclusions Klf10 is a potential biomarker in predicting and sensitizing radiation effect in pancreatic cancer.

AB - Background and purpose Krüpple-like factor 10 (Klf10), an early response gene of TGFβ, was reported to be a prognostic biomarker for pancreatic cancer survival. The role of Klf10 in predicting tumor response to cancer treatment is unknown. Materials and methods Genetically manipulated MiaPaCa and Panc-1 cells were established to evaluate clonogenic survival, autophagy, apoptosis and DNA repair after radiation. The interaction between Klf10 and UV radiation resistance-associated gene (UVRAG) was demonstrated by ChiP-PCR and luciferase reporter assay. Orthotopic murine tumor model and clinical specimens were used to evaluate radio-sensitivity of pancreatic cancer. Results We found Klf10 silencing correlates with enhanced pancreatic cancer clonogenic survival and murine tumor growth after radiation. UVRAG was an essential down-stream mediator transcriptionally suppressed by Klf10. Silencing UVRAG mRNA in Klf10 depleted Panc-1 cells reversed the radio-resistant phenotypes including decreased apoptosis and enhanced DNA repair as well as autophagy. Metformin, an anti-diabetic agent, was found to increase Klf10 and suppress UVRAG expression to improve radiation cytotoxicity in pancreatic cancer. The predictive value of Klf10 in radiation response and the inverse correlation with UVRAG were confirmed in cohorts of pancreatic cancer patients. Conclusions Klf10 is a potential biomarker in predicting and sensitizing radiation effect in pancreatic cancer.

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