Lack of modulatory function of coding nucleotide polymorphism S100A2-185G>A in oral squamous cell carcinoma

W. C. Tsai, Y. C. Lin, Sen-Tien Tsai, W. H. Shen, T. L. Chao, S. L. Lee, Li-Wha Wu

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Objective: S100A2, a Ca2+-binding protein with two EF-hands, is a tumor suppressor in oral cancer. Helix III flanking the C-terminal EF-hand is implicated to participate in the interaction of S100A2 and its target(s). The aim of this study was to examine if the coding sequence polymorphism S100A2-185G>A, leading to the peptide 62 substitution of asparagine (AAC, A allele) for serine (AGC, G allele) in helix III, had modulation effects on S100A-mediated tumor suppression. Subjects and Methods: We sequenced the coding sequence of S100A2 gene in normal oral keratinocytes (NOKs), dysplastic oral keratinocytes (DOKs), eight oral cancer lines, and 54 pairwise oral cancer specimens. We also compared the in vitro anti-tumor effect of wildtype (G allele) and variant (A allele) S100A2 expression using cell proliferation, migration, invasion, and colony formation assays. Results: With the exception of CAL27 and SCC-15 cancer lines being heterozygotes of A and G alleles, the remaining oral cells were homozygotic in G alleles. No alterations of anti-growth, anti-migration, anti-invasion, and anti-colony formation were observed between variant and wildtype cells. Moreover, no minor S100A2-185A allele was detected in 54-pairwise clinical specimens. Conclusion: The coding sequence polymorphism S100A2-185G>A had no regulatory role in S100A2-mediated tumor suppression in oral cancer.

原文English
頁(從 - 到)283-290
頁數8
期刊Oral Diseases
17
發行號3
DOIs
出版狀態Published - 2011 四月 1

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

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