Lactobacillus attenuate the progression of pancreatic cancer promoted by porphyromonas gingivalis in k-rasg12d transgenic mice

Shan Ming Chen, Li Jin Hsu, Hsiang Lin Lee, Ching Pin Lin, Szu Wei Huang, Caucasus Jun Lin Lai, Chia Wei Lin, Wan Ting Chen, Yu Jen Chen, Yu Chien Lin, Chi Chieh Yang, Ming Shiou Jan

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

Accumulating evidence suggests that there is a link between the host microbiome and pancreatic carcinogenesis, and that Porphyromonas gingivalis (P. gingivalis) increases the risk of developing pancreatic cancer. The aim of the current study was to clarify the role of P. gingivalis in the pathogenesis of pancreatic cancer and the potential immune modulatory effects of probiotics. The six-week-old LSL-K-rasG12D; Pdx-1-cre (KC) mice smeared P. gingivalis on the gums, causing pancreatic intraepithelial neoplasia (PanIN) after four weeks to be similar to the extent of lesions in untreated KC mice at 24 weeks. The oral inoculation of P. gingivalis of six-week-old LSL-K-rasG12D; Pdx-1-cre (KC) mice caused significantly pancreatic intraepithelial neoplasia (PanIN) after treatment four weeks is similar to the extent of lesions in untreated KC mice at 24 weeks. The pancreas weights of P. gingivalis plus probiotic-treated mice were significantly lower than the mice treated with P. gingivalis alone (P = 0.0028). The histological expressions of Snail-1, ZEB-1, collagen fibers, Galectin-3, and PD-L1 staining in the pancreas were also notably lower. In addition, probiotic administration reduced the histological expression of Smad3 and phosphorylated Smad3 in P. gingivalis treated KC mice. We demonstrated that oral exposure to P. gingivalis can accelerate the development of PanIN lesions. Probiotics are likely to have a beneficial effect by reducing cancer cell proliferation and viability, inhibiting PanIN progression, and cancer cell metastasis (Epithelial–mesenchymal transition, EMT). The transforming growth factor-β signaling pathway may be involved in the tumor suppressive effects of probiotics.

原文English
文章編號3522
頁(從 - 到)1-18
頁數18
期刊Cancers
12
發行號12
DOIs
出版狀態Published - 2020 12月

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

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