Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells

Tsui Ying Weng; Lien Cheng Chen; Huey Wen Shyu; Shun Hua Chen; Jen Ren Wang; Chun Keung Yu; Huan Yao Lei; Trai Ming Yeh

doi:10.1016/j.antiviral.2005.03.005

Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells

Tsui Ying Weng, Lien Cheng Chen, Huey Wen Shyu, Shun Hua Chen, Jen Ren Wang, Chun Keung Yu, Huan Yao Lei, Trai Ming Yeh

研究成果: Article › 同行評審

71 引文斯高帕斯（Scopus）

摘要

The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30 min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-α expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.

原文	English
頁（從 - 到）	31-37
頁數	7
期刊	Antiviral Research
卷	67
發行號	1
DOIs	https://doi.org/10.1016/j.antiviral.2005.03.005
出版狀態	Published - 2005 7月

All Science Journal Classification (ASJC) codes

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病毒學

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10.1016/j.antiviral.2005.03.005

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title = "Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells",

abstract = "The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30 min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-α expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.",

author = "Weng, {Tsui Ying} and Chen, {Lien Cheng} and Shyu, {Huey Wen} and Chen, {Shun Hua} and Wang, {Jen Ren} and Yu, {Chun Keung} and Lei, {Huan Yao} and Yeh, {Trai Ming}",

note = "Funding Information: This work was supported by the grant NSC93-2314-B006-116 from the National Science Council, Taiwan.",

year = "2005",

month = jul,

doi = "10.1016/j.antiviral.2005.03.005",

language = "English",

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T1 - Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells

AU - Weng, Tsui Ying

AU - Chen, Lien Cheng

AU - Shyu, Huey Wen

AU - Chen, Shun Hua

AU - Wang, Jen Ren

AU - Yu, Chun Keung

AU - Lei, Huan Yao

AU - Yeh, Trai Ming

N1 - Funding Information: This work was supported by the grant NSC93-2314-B006-116 from the National Science Council, Taiwan.

PY - 2005/7

Y1 - 2005/7

N2 - The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30 min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-α expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.

AB - The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30 min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-α expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.

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Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells

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