LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

Dhrubajyoti Das, Cheng Wen Lin, Han Sheng Chuang

研究成果: Review article同行評審

17 引文 斯高帕斯(Scopus)

摘要

Seeking optimized infectious pathogen detection tools is of primary importance to lessen the spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid-based sensing techniques, loop-mediated isothermal amplification is a promising method, as it provides rapid, sensitive, and specific detection of microbial and viral pathogens and has enormous potential to transform current point-of-care molecular diagnostics. In this review, the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined. The numerous detection methods of LAMP-based biosensors are discussed in an end-point and real-time manner with ideal examples. We also summarize the trends in LAMP-on-a-chip modalities, such as classical microfluidic, paper-based, and digital LAMP, with their merits and limitations. Finally, we provide our opinion on the future improvement of on-chip LAMP methods. This review serves as an overview of recent breakthroughs in the LAMP approach and their potential for use in the diagnosis of existing and emerging diseases.

原文English
文章編號1068
期刊Biosensors
12
發行號12
DOIs
出版狀態Published - 2022 12月

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物技術
  • 生物醫學工程
  • 儀器
  • 工程(雜項)
  • 臨床生物化學

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