Particle-laden turbulent channel flow at Reτ=644, loaded with binary mixture of particles, is numerically studied using the Lagrangian particle tracking method coupled with large eddy simulation. Turbulence statistics of different particle groups are analyzed. Two particle-wall models are applied to this study with / without considering wall roughness. Taking into considerations of rough wall model, the effect of wall roughness in the computations strengthens the wall-normal particle velocity fluctuations. As a result, particles tend to move from the near-wall region to the central core region. It leads to decrement of particle accumulation in the near-wall region as compared to the case considering the smooth wall model. The wall-normal particle mixing capability is enhanced which results in the redistribution of particles in the channel. The behavior of particle motion in the turbulent channel flow should be, thus, dependent on not only the value of Stokes number but also the wall roughness level.