Lentiviral integration preferences in transgenic mice

Shang Hsun Yang, Pei Hsun Cheng, Robert T. Sulivan, James W. Thomas, Anthony W.S. Chan

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)

摘要

Lentiviral gene transfer has a significant impact on the development of biomedical research. One of the most important features of lentiviruses is the capability to infect both dividing and nondividing cells. However, little is known whether integration preference exists, specifically in early embryos. An in-depth genome analysis on 112 independent lentiviral integration sites from 43 transgenic founder mice was performed to determine if there are preferable sites for lentiviral integration in early embryonic genome. Our results demonstrated that lentiviruses were biased in integrating within intragenic regions, especially in the introns. However, no integration preference was found associated with specific chromosomes, repetitive elements, or CpG islands, nor was there any preference for integrating at close proximity to transcription start sites. Our findings suggested that lentiviruses were biased to integrate into the intragenic regions of early embryonic genome of mouse.

原文English
頁(從 - 到)711-718
頁數8
期刊Genesis
46
發行號12
DOIs
出版狀態Published - 2008

All Science Journal Classification (ASJC) codes

  • 遺傳學
  • 內分泌
  • 細胞生物學

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