Lipid droplets are central organelles for meiosis II progression during yeast sporulation

Tzu Han Hsu, Rey Huei Chen, Yun Hsin Cheng, Chao Wen Wang, Howard Riezman

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly involving LDs and their contents. In this study, we provide cytological evidence that LDs form direct contacts with the prospore membrane (PSM) that is synthesized de novo during meiosis II to sequester the dividing nuclei in sporulating yeast. Lipidomic analyses indicate that TAG lipolysis releases free fatty acids at a time that correlates well with meiosis II progression, concomitant with phospholipid remodeling. Mutants lacking TAG or impaired of TAG hydrolysis show spore wall assembly defects, supporting a role for TAG and/or its metabolites in spore wall morphogenesis. Not only does LD integrity influence spore wall assembly, LDs are also essential for other aspects of spore development. Yeast cells lacking LDs are severely defective in PSM growth and organization and display disrupted spindles, producing dead spores or even failing to form spores. Together these results link LD physiology directly to a unique membrane morphogenesis process critical for development.

原文English
頁(從 - 到)440-451
頁數12
期刊Molecular Biology of the Cell
28
發行號3
DOIs
出版狀態Published - 2017 2月 1

All Science Journal Classification (ASJC) codes

  • 一般醫學

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