Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation

Chia Jung Kuo, Hsu Cheng Chiang, Chi Ang Tseng, Chin Fu Chang, Rajesh Kumar Ulaganathan, Tzu Ting Ling, Yu Jen Chang, Chiao Chen Chen, Yun Ru Chen, Yit Tsong Chen

研究成果: Article同行評審

16 引文 斯高帕斯(Scopus)

摘要

A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics.

原文English
頁(從 - 到)12311-12316
頁數6
期刊ACS Applied Materials and Interfaces
10
發行號15
DOIs
出版狀態Published - 2018 4月 18

All Science Journal Classification (ASJC) codes

  • 材料科學(全部)

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