Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation

Chia Jung Kuo, Hsu Cheng Chiang, Chi Ang Tseng, Chin Fu Chang, Rajesh Kumar Ulaganathan, Tzu Ting Ling, Yu Jen Chang, Chiao Chen Chen, Yun Ru Chen, Yit Tsong Chen

研究成果: Article

摘要

A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics.

原文English
頁(從 - 到)12311-12316
頁數6
期刊ACS Applied Materials and Interfaces
10
發行號15
DOIs
出版狀態Published - 2018 四月 18

指紋

Graphite
Field effect transistors
Amyloid
Biosensors
Graphene
Lipids
Agglomeration
Monitoring
Cell membranes
Kinetics
Assays
Graphene transistors
Proteins
G(M1) Ganglioside
Phospholipase D
Lipid bilayers
Cholera Toxin
Biomimetics
Hydrolysis
Nucleation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

引用此文

Kuo, C. J., Chiang, H. C., Tseng, C. A., Chang, C. F., Ulaganathan, R. K., Ling, T. T., ... Chen, Y. T. (2018). Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation. ACS Applied Materials and Interfaces, 10(15), 12311-12316. https://doi.org/10.1021/acsami.8b01917
Kuo, Chia Jung ; Chiang, Hsu Cheng ; Tseng, Chi Ang ; Chang, Chin Fu ; Ulaganathan, Rajesh Kumar ; Ling, Tzu Ting ; Chang, Yu Jen ; Chen, Chiao Chen ; Chen, Yun Ru ; Chen, Yit Tsong. / Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation. 於: ACS Applied Materials and Interfaces. 2018 ; 卷 10, 編號 15. 頁 12311-12316.
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title = "Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation",
abstract = "A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics.",
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Kuo, CJ, Chiang, HC, Tseng, CA, Chang, CF, Ulaganathan, RK, Ling, TT, Chang, YJ, Chen, CC, Chen, YR & Chen, YT 2018, 'Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation', ACS Applied Materials and Interfaces, 卷 10, 編號 15, 頁 12311-12316. https://doi.org/10.1021/acsami.8b01917

Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation. / Kuo, Chia Jung; Chiang, Hsu Cheng; Tseng, Chi Ang; Chang, Chin Fu; Ulaganathan, Rajesh Kumar; Ling, Tzu Ting; Chang, Yu Jen; Chen, Chiao Chen; Chen, Yun Ru; Chen, Yit Tsong.

於: ACS Applied Materials and Interfaces, 卷 10, 編號 15, 18.04.2018, p. 12311-12316.

研究成果: Article

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AU - Kuo, Chia Jung

AU - Chiang, Hsu Cheng

AU - Tseng, Chi Ang

AU - Chang, Chin Fu

AU - Ulaganathan, Rajesh Kumar

AU - Ling, Tzu Ting

AU - Chang, Yu Jen

AU - Chen, Chiao Chen

AU - Chen, Yun Ru

AU - Chen, Yit Tsong

PY - 2018/4/18

Y1 - 2018/4/18

N2 - A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics.

AB - A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (Aβ40) aggregation. In the early nucleation stage of Aβ40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the Aβ40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of Aβ40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics.

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Kuo CJ, Chiang HC, Tseng CA, Chang CF, Ulaganathan RK, Ling TT 等. Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-β Aggregation. ACS Applied Materials and Interfaces. 2018 4月 18;10(15):12311-12316. https://doi.org/10.1021/acsami.8b01917