Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles

Chien-Sheng Chen, Jie Yao, Richard A. Durst

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy was used to measure the average number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. Each liposome encapsulated an average of three QDs. Our work demonstrated that nanoparticles could be successfully encapsulated into liposomes and provided a methodology to measure the nanoparticle quantity inside each liposome with fluorescence correlation spectroscopy.

原文English
主出版物標題2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
編輯M. Laudon, B. Romanowicz
頁面206-209
頁數4
出版狀態Published - 2005 十二月 1
事件2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 - Anaheim, CA, United States
持續時間: 2005 五月 82005 五月 12

出版系列

名字2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings

Other

Other2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005
國家United States
城市Anaheim, CA
期間05-05-0805-05-12

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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  • 引用此

    Chen, C-S., Yao, J., & Durst, R. A. (2005). Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles. 於 M. Laudon, & B. Romanowicz (編輯), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings (頁 206-209). (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).