Live cell tracking based on cellular state recognition from microscopic images

Y. N. Sun, C. H. Lin, C. C. Kuo, C. L. Ho, C. J. Lin

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

The analysis of cell motion is an essential process in fundamental medical studies because most active cellular functions involve motion. In this paper, a computer-assisted motion analysis system is proposed for cell tracking. In the proposed tracking process, unlike in conventional tracking methods, cellular states referring to the cellular life cycle are defined and appropriate strategies are adopted for cells at different states. The use of cellular state recognition allows detection of possible cell division and hence can improve the robustness of cell tracking. Experimental results show that cells can be successfully segmented and tracked over a long period of time, and the proposed system is found to be as accurate as manual tracking. Various quantitative analyses and visualizations are used to represent cell motion, which demonstrates the usefulness of the proposed system in the study of cell dynamics.

原文English
頁(從 - 到)94-105
頁數12
期刊Journal of Microscopy
235
發行號1
DOIs
出版狀態Published - 2009 7月

All Science Journal Classification (ASJC) codes

  • 病理學與法醫學
  • 組織學

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