Mab_3083c is a homologue of RNase J and plays a role in colony morphotype, aggregation, and sliding motility of mycobacterium abscessus

Ting Yu Liu, Sheng Hui Tsai, Jenn Wei Chen, Yu Ching Wang, Shiau Ting Hu, Yih Yuan Chen

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摘要

Mycobacterium abscessus is an opportunistic pathogen causing human diseases, especially in immunocompromised patients. M. abscessus strains with a rough morphotype are more virulent than those with a smooth morphotype. Morphotype switch may occur during a clinical infection. To investigate the genes involved in colony morphotype switching, we performed transposon mutagenesis in a rough clinical strain of M. abscessus. A morphotype switching mutant (smooth) named mab_3083c::Tn was obtained. This mutant was found to have a lower aggregative ability and a higher sliding motility than the wild type strain. However, its glycopeptidolipid (GPL) content remained the same as those of the wild type. Complementation of the mutant with a functional mab_3083c gene reverted its morphotype back to rough, indicating that mab_3083c is associated with colony morphology of M. abscessus. Bioinformatic analyses showed that mab_3083c has a 75.4% identity in amino acid sequence with the well-characterized ribonuclease J (RNase J) of M. smegmatis (RNase JMsmeg). Complementation of the mutant with the RNase J gene of M. smegmatis also switched its colony morphology from smooth back to rough. These results suggest that Mab_3083c is a homologue of RNase J and involved in regulating M. abscessus colony morphotype switching.

原文English
文章編號676
期刊Microorganisms
9
發行號4
DOIs
出版狀態Published - 2021

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology
  • Microbiology (medical)

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