Magnesium Sulfate Diminishes the Effects of Amide Local Anesthetics in Rat Sciatic-Nerve Block

Yu Chun Hung, Chia Ying Chen, Philipp Lirk, Chi-Fei Wang, Jen Kun Cheng, Chien Chuan Chen, Ging Kuo Wang, Peter Gerner

研究成果: Article

23 引文 (Scopus)

摘要

Background and Objectives: Magnesium sulfate (MgSO4) is well known as an antagonist of N-methyl-d-aspartate receptors and was used for intrathecal analgesia a century ago. However, the effects of MgSO4 combined with local anesthetics (LAs) on peripheral nerves are unclear. We tested the hypothesis that MgSO4 could be used as an adjuvant to prolong and intensify conduction block by amide-type LAs in a rat sciatic-nerve block model. Further, the mechanism of possible synergy between LAs and MgSO4 was investigated in whole-cell mode patch-clamp experiments. Methods: Sciatic nerves were exposed to 2%/73.9 mM lidocaine, 0.25%/7.7 mM bupivacaine, and 0.5%/15.4 mM ropivacaine, with or without addition of 1.25%, 2.5%, or 5% MgSO4/50.7 mM, and nerve block characteristics were assessed. To elucidate the LA-MgSO4 interaction, voltage-dependent inactivation curves were determined in cultured rat GH3 cells that expressed neuronal Na+ channels. Results: Unexpectedly, the addition of MgSO4 overall significantly shortened the duration of block by lidocaine, bupivacaine, and ropivacaine. The steady-state inactivation of Na+ channels in the presence of 300 μM lidocaine was almost unchanged by the addition of 10 mM MgSO4, indicating that MgSO4 does not affect the potency of lidocaine toward the inactivated Na+ channel. Conclusions: MgSO4 coadministered with amide-type LAs shortened the duration of sciatic-nerve block in rats. Therefore, it does not seem to be useful as an adjuvant for peripheral-nerve block. The mechanism of this observed antagonism is unclear but appears to be independent of the action of LAs and MgSO4 at the LA receptor within the Na+ channel.

原文English
頁(從 - 到)288-295
頁數8
期刊Regional anesthesia and pain medicine
32
發行號4
DOIs
出版狀態Published - 2007 七月 1

指紋

Magnesium Sulfate
Nerve Block
Sciatic Nerve
Local Anesthetics
Amides
Lidocaine
Bupivacaine
Peripheral Nerves
Analgesia

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

引用此文

Hung, Yu Chun ; Chen, Chia Ying ; Lirk, Philipp ; Wang, Chi-Fei ; Cheng, Jen Kun ; Chen, Chien Chuan ; Wang, Ging Kuo ; Gerner, Peter. / Magnesium Sulfate Diminishes the Effects of Amide Local Anesthetics in Rat Sciatic-Nerve Block. 於: Regional anesthesia and pain medicine. 2007 ; 卷 32, 編號 4. 頁 288-295.
@article{412d1e39f1ed4f1babcc1e442da56707,
title = "Magnesium Sulfate Diminishes the Effects of Amide Local Anesthetics in Rat Sciatic-Nerve Block",
abstract = "Background and Objectives: Magnesium sulfate (MgSO4) is well known as an antagonist of N-methyl-d-aspartate receptors and was used for intrathecal analgesia a century ago. However, the effects of MgSO4 combined with local anesthetics (LAs) on peripheral nerves are unclear. We tested the hypothesis that MgSO4 could be used as an adjuvant to prolong and intensify conduction block by amide-type LAs in a rat sciatic-nerve block model. Further, the mechanism of possible synergy between LAs and MgSO4 was investigated in whole-cell mode patch-clamp experiments. Methods: Sciatic nerves were exposed to 2{\%}/73.9 mM lidocaine, 0.25{\%}/7.7 mM bupivacaine, and 0.5{\%}/15.4 mM ropivacaine, with or without addition of 1.25{\%}, 2.5{\%}, or 5{\%} MgSO4/50.7 mM, and nerve block characteristics were assessed. To elucidate the LA-MgSO4 interaction, voltage-dependent inactivation curves were determined in cultured rat GH3 cells that expressed neuronal Na+ channels. Results: Unexpectedly, the addition of MgSO4 overall significantly shortened the duration of block by lidocaine, bupivacaine, and ropivacaine. The steady-state inactivation of Na+ channels in the presence of 300 μM lidocaine was almost unchanged by the addition of 10 mM MgSO4, indicating that MgSO4 does not affect the potency of lidocaine toward the inactivated Na+ channel. Conclusions: MgSO4 coadministered with amide-type LAs shortened the duration of sciatic-nerve block in rats. Therefore, it does not seem to be useful as an adjuvant for peripheral-nerve block. The mechanism of this observed antagonism is unclear but appears to be independent of the action of LAs and MgSO4 at the LA receptor within the Na+ channel.",
author = "Hung, {Yu Chun} and Chen, {Chia Ying} and Philipp Lirk and Chi-Fei Wang and Cheng, {Jen Kun} and Chen, {Chien Chuan} and Wang, {Ging Kuo} and Peter Gerner",
year = "2007",
month = "7",
day = "1",
doi = "10.1016/j.rapm.2007.03.008",
language = "English",
volume = "32",
pages = "288--295",
journal = "Regional Anesthesia and Pain Medicine",
issn = "1098-7339",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

Magnesium Sulfate Diminishes the Effects of Amide Local Anesthetics in Rat Sciatic-Nerve Block. / Hung, Yu Chun; Chen, Chia Ying; Lirk, Philipp; Wang, Chi-Fei; Cheng, Jen Kun; Chen, Chien Chuan; Wang, Ging Kuo; Gerner, Peter.

於: Regional anesthesia and pain medicine, 卷 32, 編號 4, 01.07.2007, p. 288-295.

研究成果: Article

TY - JOUR

T1 - Magnesium Sulfate Diminishes the Effects of Amide Local Anesthetics in Rat Sciatic-Nerve Block

AU - Hung, Yu Chun

AU - Chen, Chia Ying

AU - Lirk, Philipp

AU - Wang, Chi-Fei

AU - Cheng, Jen Kun

AU - Chen, Chien Chuan

AU - Wang, Ging Kuo

AU - Gerner, Peter

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Background and Objectives: Magnesium sulfate (MgSO4) is well known as an antagonist of N-methyl-d-aspartate receptors and was used for intrathecal analgesia a century ago. However, the effects of MgSO4 combined with local anesthetics (LAs) on peripheral nerves are unclear. We tested the hypothesis that MgSO4 could be used as an adjuvant to prolong and intensify conduction block by amide-type LAs in a rat sciatic-nerve block model. Further, the mechanism of possible synergy between LAs and MgSO4 was investigated in whole-cell mode patch-clamp experiments. Methods: Sciatic nerves were exposed to 2%/73.9 mM lidocaine, 0.25%/7.7 mM bupivacaine, and 0.5%/15.4 mM ropivacaine, with or without addition of 1.25%, 2.5%, or 5% MgSO4/50.7 mM, and nerve block characteristics were assessed. To elucidate the LA-MgSO4 interaction, voltage-dependent inactivation curves were determined in cultured rat GH3 cells that expressed neuronal Na+ channels. Results: Unexpectedly, the addition of MgSO4 overall significantly shortened the duration of block by lidocaine, bupivacaine, and ropivacaine. The steady-state inactivation of Na+ channels in the presence of 300 μM lidocaine was almost unchanged by the addition of 10 mM MgSO4, indicating that MgSO4 does not affect the potency of lidocaine toward the inactivated Na+ channel. Conclusions: MgSO4 coadministered with amide-type LAs shortened the duration of sciatic-nerve block in rats. Therefore, it does not seem to be useful as an adjuvant for peripheral-nerve block. The mechanism of this observed antagonism is unclear but appears to be independent of the action of LAs and MgSO4 at the LA receptor within the Na+ channel.

AB - Background and Objectives: Magnesium sulfate (MgSO4) is well known as an antagonist of N-methyl-d-aspartate receptors and was used for intrathecal analgesia a century ago. However, the effects of MgSO4 combined with local anesthetics (LAs) on peripheral nerves are unclear. We tested the hypothesis that MgSO4 could be used as an adjuvant to prolong and intensify conduction block by amide-type LAs in a rat sciatic-nerve block model. Further, the mechanism of possible synergy between LAs and MgSO4 was investigated in whole-cell mode patch-clamp experiments. Methods: Sciatic nerves were exposed to 2%/73.9 mM lidocaine, 0.25%/7.7 mM bupivacaine, and 0.5%/15.4 mM ropivacaine, with or without addition of 1.25%, 2.5%, or 5% MgSO4/50.7 mM, and nerve block characteristics were assessed. To elucidate the LA-MgSO4 interaction, voltage-dependent inactivation curves were determined in cultured rat GH3 cells that expressed neuronal Na+ channels. Results: Unexpectedly, the addition of MgSO4 overall significantly shortened the duration of block by lidocaine, bupivacaine, and ropivacaine. The steady-state inactivation of Na+ channels in the presence of 300 μM lidocaine was almost unchanged by the addition of 10 mM MgSO4, indicating that MgSO4 does not affect the potency of lidocaine toward the inactivated Na+ channel. Conclusions: MgSO4 coadministered with amide-type LAs shortened the duration of sciatic-nerve block in rats. Therefore, it does not seem to be useful as an adjuvant for peripheral-nerve block. The mechanism of this observed antagonism is unclear but appears to be independent of the action of LAs and MgSO4 at the LA receptor within the Na+ channel.

UR - http://www.scopus.com/inward/record.url?scp=34548039016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548039016&partnerID=8YFLogxK

U2 - 10.1016/j.rapm.2007.03.008

DO - 10.1016/j.rapm.2007.03.008

M3 - Article

C2 - 17720112

AN - SCOPUS:34548039016

VL - 32

SP - 288

EP - 295

JO - Regional Anesthesia and Pain Medicine

JF - Regional Anesthesia and Pain Medicine

SN - 1098-7339

IS - 4

ER -