Manipulation and impedance measurement of single HeLa cells based on the dieletrophoresis microwell

Kung Chieh Lan, Ming Kun Chen, Chia Feng Liu, Ling Sheng Jang

研究成果: Conference contribution

摘要

The research of individual cells is an important technique in many kinds of biological study. In addition, cell impedance analysis is an effective way of biological measurement. Therefore, integrating the measurement technique into the single-cell trapping structure is the aim of this work. This study presents a cell manipulation and measurement microchip to achieve a precise positioning which uses the Alternating current electrokinetics (ACET) and negative dielectrophoresis (nDEP) force to move the particle and cell on the measurement electrodes. ACET and DEP are the electrical methods to manipulate particle, and can be easily combined with subsequent analyses based on electric fields. The microwell in the prior study is separated into two parts and regarded as the measurement electrodes. Besides, the original structure has been modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the parameters in the structure. By means of simulations and analyses, the optimum structure for the Hela cell is presented. Finally, the structure was applied to trap and measure the HeLa cell.

原文English
主出版物標題NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
頁面79-83
頁數5
DOIs
出版狀態Published - 2011
事件6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
持續時間: 2011 二月 202011 二月 23

出版系列

名字NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
國家/地區Taiwan
城市Kaohsiung
期間11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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