Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system

Ko Wei Chang, Jing Lun Li, Ching Hsuan Yang, Shu Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

4 引文 斯高帕斯(Scopus)

摘要

Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.

原文English
主出版物標題9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
發行者Institute of Electrical and Electronics Engineers Inc.
頁面647-650
頁數4
ISBN(電子)9781479947270
DOIs
出版狀態Published - 2014 九月 23
事件9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States
持續時間: 2014 四月 132014 四月 16

出版系列

名字9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Other

Other9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
國家/地區United States
城市Waikiki Beach
期間14-04-1314-04-16

All Science Journal Classification (ASJC) codes

  • 控制與系統工程
  • 電氣與電子工程

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