Mechanical quantification of the epithelial mesenchymal transition

Tsung Hsien Wu, Jen I. Liang, Yu Wei Chiu, Ming Long Yeh, Chia Hsin Chen

研究成果: Conference contribution

摘要

During EMT (Epithelial mesenchymal transition), cells are under large shape change. The ingress of the cell relies on the debonding between adjacent epithelium and the flexibility change of them. In order to elucidate the mechanical involvement of EMT, it is necessary to investigate the mechanical properties, cell compressive stiffness and extensibility and the adhesion strength between them. The purpose of this study was using special micropipette to quantify the mechanical property transition when the cell type changed. We built a new special micropipette system which could be used to measure both the tension and compression rigidities of cells before and after EMT. We changed the original design of dual micropipettes with strain gauge cell mechanical system into single micropipette with AFM Cantilever tip for force quantification. We had finished the build up of the micropipette system and AFM tip holder with electronic manipulator. The normal mouse mammary gland cells (NMuMGs) had been successfully grabbed by this novel micropipette system and the force displacement curve for axial compression test of NMuMG had been recorded. However, like the most in house design machine, this novel micropipette mechanical system needs further verification in accuracy and calibration.

原文English
主出版物標題NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
頁面458-461
頁數4
DOIs
出版狀態Published - 2011 十月 4
事件6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
持續時間: 2011 二月 202011 二月 23

出版系列

名字NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
國家/地區Taiwan
城市Kaohsiung
期間11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

指紋

深入研究「Mechanical quantification of the epithelial mesenchymal transition」主題。共同形成了獨特的指紋。

引用此