Mechanism of nanoformulated graphene oxide-mediated human neutrophil activation

Yu Jen Lu, Yi Hsuan Wang, Rama Shanker Sahu, Jyh Ping Chen, Banendu Sunder Dash, Pei Jen Chung, Hung Wei Yang, Er Yuan Chuang, Tsong Long Hwang

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)


Understanding the molecular mechanisms of graphene oxide (GO)-based biomaterials is important for logical biomedical applications. Previous studies have revealed biointeractions between GO and immune effector cells, but the effects on neutrophils, crucial cells in the immune system, have not been thoroughly discussed. In this study, GO nanoformulations were synthesized with different functional groups, including GO, GO-carboxylated (GO-COOH), and PEGylated GO (GO-PEG), with different surface features, which were elucidated using imaging methods and surface-sensitive quantitative spectroscopic techniques, including atomic force microscopy (AFM), transmission electron microscopy (TEM), and X-ray photoemission spectroscopy (XPS). The GO-based nanoformulations elicited reactive oxygen species (ROS) generation and neutrophil extracellular trap (NET) formation in human neutrophils. Nanoformulated GO stimulates NET development via the formation of ROS. An endocytosis study revealed that nanoformulated GO facilitated internalization by neutrophils via macropinocytosis and actin-dependent phagocytosis. Importantly, calcium mobilization and phosphorylation proteins such as mitogen-Activated protein kinases (extracellular signal-regulated kinase, c-Jun N-Terminal kinase, and p38) and AKT were involved in the activation of neutrophils. These findings offer the first verification that nanoformulated GO exhibits direct effects on human neutrophils.

頁(從 - 到)40141-40152
期刊ACS Applied Materials and Interfaces
出版狀態Published - 2020 9月 9

All Science Journal Classification (ASJC) codes

  • 材料科學(全部)


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