Mechanism of two-photon fluorescence increment via crosslinked bovine serum albumin

C. Y. Lin, C. H. Lien, C. Y. Chang, S. J. Chen

研究成果: Conference contribution

摘要

The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.

原文English
主出版物標題Physical Chemistry of Interfaces and Nanomaterials XI
DOIs
出版狀態Published - 2012 十二月 1
事件Physical Chemistry of Interfaces and Nanomaterials XI - San Diego, CA, United States
持續時間: 2012 八月 122012 八月 15

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
8459
ISSN(列印)0277-786X

Conference

ConferencePhysical Chemistry of Interfaces and Nanomaterials XI
國家/地區United States
城市San Diego, CA
期間12-08-1212-08-15

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 電腦科學應用
  • 應用數學
  • 電氣與電子工程

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