Mechanisms of silver nanoparticle-induced toxicity and important role of autophagy

Bin Hsu Mao, Jui-Chen Tsai, Chun Wan Chen, Shian-Jang Yan, Ying-Jan Wang

研究成果: Review article

44 引文 (Scopus)

摘要

Safety concerns have been raised over the extensive applications of silver nanoparticles (AgNPs) because nano dimensions make them highly bioactive, being potentially harmful to the exposed humans. Surface physico-chemistry (shape, surface charge, chemical composition, etc.) that mainly dictates nano-bio interactions is relevant for influencing their biocompatibility and toxicity. Although the hazardousness of AgNPs has been demonstrated in vitro and in vivo, mechanistic understanding of the toxicity particularly at the molecular and organismal levels, in addition to oxidative stress and silver ion dissolution, has remained unclear. A growing body of research has elucidated that autophagy, being activated in response to exposure to various nanomaterials, may serve as a cellular defense mechanism against nanotoxicity. Recently, autophagy activation was shown to correlate with AgNPs exposure; however, the subsequent autophagosome–lysosome fusion was defective. As autophagy plays a crucial role in selective removal of stress-mediated protein aggregates and injured organelles, AgNPs-induced autophagic flux defect may consequently lead to aggravated cytotoxic responses. Furthermore, we suggest that p62 accumulation resulting from defective autophagy may also potentially account for AgNPs cytotoxicity. Intriguingly, AgNPs have been shown to interfere with ubiquitin modifications, either via upregulating levels of enzymes participating in ubiquitination, or through impairing the biological reactivity of ubiquitin (due to formation of AgNPs-ubiquitin corona). Ubiquitination both confers selectivity to autophagy as well as modulates stabilization, activation, and trafficking of proteins involved in autophagic clearance pathways. In this regard, we offer a new perspective that interference of AgNPs with ubiquitination may account for AgNPs-induced defective autophagy and cytotoxic effects.

原文English
頁(從 - 到)1021-1040
頁數20
期刊Nanotoxicology
10
發行號8
DOIs
出版狀態Published - 2016 九月 13

指紋

Autophagy
Ubiquitin
Silver
Nanoparticles
Toxicity
Chemical activation
Proteins
Oxidative stress
Ubiquitination
Cytotoxicity
Surface charge
Surface chemistry
Biocompatibility
Nanostructured materials
Dissolution
Fusion reactions
Enzymes
Stabilization
Fluxes
Defects

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Toxicology

引用此文

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Mechanisms of silver nanoparticle-induced toxicity and important role of autophagy. / Mao, Bin Hsu; Tsai, Jui-Chen; Chen, Chun Wan; Yan, Shian-Jang; Wang, Ying-Jan.

於: Nanotoxicology, 卷 10, 編號 8, 13.09.2016, p. 1021-1040.

研究成果: Review article

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AU - Mao, Bin Hsu

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